重症急性胰腺炎致急性肺损伤动物模型血气分析及形态学研究  

Blood gas analysis and morphology study of animals with acute lung injury caused by severe acute pancreatitis

作　　者：金健[1] 刘斌[1] 雷跃昌[1] 胡敬国[1] 王革平[1] 李霭建[1] 刘扬[1] 

机构地区：[1]成都大学附属医院胸外科,四川成都610081

出　　处：《临床肺科杂志》2013年第2期251-253,共3页Journal of Clinical Pulmonary Medicine

摘　　要：目的观察SD大鼠重症急性胰腺炎致急性肺损伤后动脉血气分析及形态学改变。方法健康成年SD大鼠20只,随机分成两组,A组10只,注射同等剂量的生理盐水;B组10只,腹腔注射脂多糖建立重症急性胰腺炎的动物模型,观察病理学改变。结果血气分析变化:①PaO2:伤后12～48 h两组SD大鼠相比较有差异(P<0.05),B组低于A组。②PaCO2:两组SD大鼠伤后12～48 h相比较均有差异(P<0.01),B组高于A组。病理学改变:B组急性肺损伤表现(中性粒细胞浸润、肺泡内炎性物质渗出),随着时间延长,视野内中性粒细胞增多。结论动脉血气指标PaO2、PaCO2在重症急性胰腺炎致急性肺损伤后可用作为判断肺组织损伤严重程度的参考指标,PaO2水平能反映肺损伤程度,在伤后24 h～48 h检测的指标更明显。Objective To investigate arterial blood gas analysis and morphological changes of Sprague-Dawley （SD） rats with acute lung injury caused by severe acute pancreatitis. Methods 20 healthy adult SD rats were randomly and evenly divided into two groups. The group A were injected with a certain dose of saline as the controls, and the group B were injected with LPS through abdominal cavity to establish an animal model of acute lung injury caused by severe acute pancreatitis. The physiopathology changes were investiga- ted. Results The blood gas analysis showed： the level of PaO2 in the group A was higher than that in the group B after 12 -48 hours with statistical difference （ P 〈 0. 05 ）, and the level of PaCO2 in the group A was lower than that in the group B with statistical difference （P 〈0. 01 ）. The pathological changes showed that the number of neutrophilic granulocyte increased along with time expand in the group B. Conclusion The levels of PaO2 and PaCO2 can clearly reflect the severity of lung injury caused by severe acute pancreatitis after 24 -48 h.

关 键 词：重症急性胰腺炎 急性肺损伤 动物实验替代试验 血气分析 肺 病理生理学 

分 类 号：R576[医药卫生—消化系统] R-332[医药卫生—内科学]