汉防己甲素对人大肠癌细胞株的放射增敏作用  

Reseach on radiosensitivity by tetrandrine in human colonrectal cancer cells

作　　者：方明治[1] 李敏[1] 曹远东[2] 成红艳[2] 

机构地区：[1]南京市中医院肿瘤科,江苏南京210001 [2]东南大学附属中大医院肿瘤科,江苏南京210009

出　　处：《现代肿瘤医学》2013年第1期24-27,共4页Journal of Modern Oncology

基　　金：江苏省卫生科技项目(编号:H200845)

摘　　要：目的:研究汉防己甲素(Tet)对人大肠癌细胞SW620的放射增敏作用并初步探讨其作用机制。方法:采用MTT药敏试验、克隆形成分析法检测Tet对SW620细胞的放射增敏作用,流式细胞仪检测细胞周期、细胞凋亡情况。结果:不同浓度的Tet作用于人SW620细胞株后,其细胞毒性呈剂量依赖性(P<0.05)。Tet对SW620细胞的72h半数抑制浓度(IC50)为:18.359μg/ml。Tet(5μg/ml)能明显降低放射后SW620的克隆形成率,增加X射线的杀伤作用,其放射增敏比SER(Dq)为1.6。流式细胞术结果显示,Tet能够导致放射后SW620细胞G2/M期阻滞,同时增加照射后细胞凋亡比。结论:Tet对SW620细胞株有放射增敏作用,其作用机制可能与Tet阻滞细胞于G2/M期,增加凋亡有关。Objective ：To investigate the radiosensitization of tetrandrine on colonrectal cancer cell line SW620 and its possible mechanism. Methods ： MTr assay was used to measure the effect of tetrandrine on SW620 cell prolifera- tion. Clonogenic assay was performed to determine the radiosensitizing effect of tetrandrine on SW620 cell line. Flow cytometry detect cell cycle and apoptosis. Results： Tet in a dose - dependent manner inhibited SW620 cell line prolif- eration （ P 〈 0.05 ）, the ICso of Tet on SW620 for 72 hours was 18. 359 μg/ml. Tet （ 5 μg/ml） decreased the Clone - forming rate,increased the oneolytic effect. The radiosensitization enhancement ratios was 1.6. The radiosensitization group had a higher apoptosis rate and GE/M phase cells. Condusion：Tet could enhance the radiosentivity of human colonrectal cancer cell line SW620,the effect mechanism possibly associated with encouraging apoptosis and increas- ing G2/M phase cells.

关 键 词：肠癌 汉防己甲素 放射敏感性 

分 类 号：R730.5[医药卫生—肿瘤] R735.34[医药卫生—临床医学]