山仙颗粒含药血清诱导S-180肉瘤细胞凋亡及其对bcl-2、Bax基因表达的影响  

Effect of rat serum with SXG on apoptosis and the expressions of bcl-2/Bax in S-180 cells

作　　者：应小平[1] 吴肖晓[1] 方艳[1] 胥冰[1] 王小平[1] 郭兰生[1] 赵延红[1] 

机构地区：[1]陕西中医学院病理学教研室,陕西咸阳712046

出　　处：《现代肿瘤医学》2013年第1期28-30,共3页Journal of Modern Oncology

基　　金：陕西省科技厅自然科学基础研究项目(编号:SJ08C229)

摘　　要：目的:探讨山仙颗粒(SXG)含药血清诱导体外培养S-180肉瘤细胞的凋亡作用及其对凋亡相关基因bcl-2及Bax表达的影响。方法:以不同浓度的山仙颗粒含药血清作用于体外培养的S-180肉瘤细胞,待24、48、72h后倒置显微镜下观察细胞形态变化并制备细胞涂片,采用TUNEL法检S-180肉瘤细胞凋亡率,免疫细胞化学(SABC)检测S-180肉瘤细胞中凋亡相关基因bcl-2、Bax表达的变化。结果:SXG含药血清可明显诱导S-180肉瘤细胞凋亡,导致其细胞形态改变,并能使bcl-2表达降低(P<0.01)、Bax表达增强(P<0.01),且呈浓度和时间依赖性。结论:SXG含药血清能有效诱体外培养的S-180肉瘤细胞凋亡,其机制可能与抑制bcl-2表达,促进Bax表达有关。Objective： To investigate the effects of the serum containing ShaM Xian Granule （SXG） on apoptosis and the apoptosis related genes bcl- 2 and Bax express in S- 180 cell. Methods：With different concentrations of rat serum containing SXG effect on S - 180 cultured in RPMI1640, after 24,48,72 hours to observe the change of cell morphology under microscope. The apoptosis of S - 180 was detected by TUNEL and the bcl -2, Bax gene expression levels were detected by immunohistochemistry（SABC）. Results ：The rat serum containing SXG can induce apoptosis of S - 180 and change the morphology of S - 180. It can decrease bcl - 2 gene expressed obviously （ P 〈 0.01 ） and the Bax gene expression increased significantly（P 〈 0.01 ）. Conclusion：The rat serum containing SXG can induce apop- tosis of S - 180 in vitro,which maybe related with decreased bcl -2 gene express and increased Bax gene expression.

关 键 词：山仙颗粒 S-180肉瘤细胞 细胞凋亡 bcl-2 BAX 

分 类 号：R73-361[医药卫生—肿瘤] R738.6[医药卫生—临床医学]