MDR1基因的表达与肝癌生物学行为指标的关系  

Relationship between the expression of MDR1 in hepatocellular cancer and its biological behaviours

作　　者：李海平[1] 高波[1] 余宗涛[1] 吕军[1] 李文翠[1] 张吉才[1] 

机构地区：[1]湖北医药学院附属太和医院输血科,湖北十堰442000

出　　处：《现代肿瘤医学》2013年第1期129-132,共4页Journal of Modern Oncology

摘　　要：目的:通过对肝癌(hepatocellular carcinoma,HCC)中多药耐药基因(multid-rug resistance gene 1,MDR1)表达和生物学行为指标HBV、AFP、AST的检测,探讨肝癌中MDR1的表达特点及其与肝癌生物学行为的相互关系。方法:运用荧光定量PCR(flurescence quantitative-PCR,FQ-PCR)技术检测MDR1基因在51例肝癌组织和10例正常肝组织中的表达,分析MDR1基因的表达与肝癌生物学特性之间的关系。结果:MDR1在肝癌中的表达为0.55±0.27,正常肝组织中的表达为0.23±0.10,(P<0.05)。表达与肿瘤Ed-mondson分级呈正相关(P<0.05)。HBV感染、AFP阳性、AST升高与MDR1基因表达明显呈正相关(r=0.463、0.473、0.299)。结论:肝癌中存在原发性耐药的现象,MDR1基因高表达在肝癌原发性耐药中可能起重要作用;HBV的检测可以作为肝癌化疗耐药的参考指标之一,血浆中AFP水平、AST变化可以作为动态监测MDR1表达的参考指标。Objective：To detect the expression of MDR1 gene and HBV, AFP in hepatocellular cancer （HCC） and evaluate the relationship between the expression of MDR1 and HBV infection, AFP and AST level. Methods： FQ - PCR was used to examine the expression of MDR1 in 51 HCC cases and 10 normal tissues. Results：The expres- sions of MDR1 mRNA were 0.55±0. 27 in HCC and 0.23 0. 10 in normal hepatic - tissues, the expressions of MDR1 in HCC were higher than those in normal tissues （ P 〈 0.05 ）. And the expression of gene was positively related to tumor Edmondson grade（P 〈 0.05 ）. The expression of MDR1 was correlated with HBV infection. AFP and AST level. Conclusion：Primary resistance exsisted in HCC; higher expression of MDR1 may play a major role in the in- trinsic MDR of HCC. The state of HBV infection maybe a useful standard in chemotherapy resistance , AFP and AST can be regarded as valuable indicators for the expression of MDR1 gene in HCC.

关 键 词：多药耐药基因 肝癌 HBV感染 AFP 表达 

分 类 号：R735.7[医药卫生—肿瘤]