癌光啉对人乳腺癌细胞MDA-MB-231的光动力杀伤效应及其机制研究  

Photodynamic damage of carcinophotorin on breast cancer cells MDA-MB-231 and its mechanism

作　　者：陈磊[1] 孔祥杰[1] 李佳[1] 张俊峰[1] 房林[1] 

机构地区：[1]上海市第十人民医院甲乳外科,上海市200072

出　　处：《中国肿瘤临床》2013年第1期3-7,共5页Chinese Journal of Clinical Oncology

摘　　要：目的:探讨癌光啉(PSD-007)在体外对于乳腺癌细胞株MDA-MB-231光动力杀伤效应,并分析其分子机制。方法:MTT法检测不同浓度PSD-007(0、2、4、6、8、10μg/mL)作用于MDA-MB-231细胞株后对其增殖的影响;光学显微镜下观察光动力治疗后细胞形态的变化,荧光显微镜分析PSD-007作用后细胞的死亡形式。应用RT-PCR和Western blotting技术分析其分子机制。结果:当PSD007浓度为10μg/mL、光照能量为9.0 J/cm2时,对乳腺癌细胞的光动力杀伤效应最大,抑制率为97.01%。光动力治疗处理后的细胞逐渐变圆,体积变小,核质比增大,折光性减弱,贴壁能力下降,细胞间隙增大,直到细胞漂浮死亡。荧光显微镜分析结果显示,光动力治疗后死亡细胞主要为坏死或晚期的凋亡细胞。RT-PCR和Western blot结果显示相比对照组,实验组Caspase3、Caspase8、P65亚基和P53表达水平明显上调,而Bcl-2和Bcl-x表达无明显改变。结论:PSD-007在体外通过调控Caspase蛋白酶、P53、NF-KB通路对人乳腺癌MDA-MB-231细胞具有光动力杀伤效应,可能成为未来乳腺癌治疗的一个新方式。Objective： This study aims to evaluate the efficacy of carcinophotorin （PSD-007） photosensitization on the apopto- sis-induced response in human breast cancer cells and analyze the mechanisms of PSD-007 involved in this process. Methods： Methyl thiazolyl tetrazolium （MTT） assay and in situ labeling were performed to examine the effects of the photodynamic therapy （PDT） on the proliferation and apoptosis of the cancer cells MDA-MB-231, respectively. Changes in cellular morphology were assessed using an optical microscope. Real-time polymerase chain reaction and Western blot analysis were conducted to clarify the underlying mecha- nisms. Results： The MTT assay revealed that at a concentration of 10 μg/mL and in combination with 9.0 J/cm2 laser radiation power 24 h after cell culture, PSD-007 markedly inhibited the proliferation of breast tumor cells, with an inhibition rate of 97.01%. Under a fluorescent microscope, apoptotic cells in the treatment groups with 5 and 10 μg/mL PSD-007-PDT were observed to have dramatically outnumbered the control groups. The dead cells after PSD-007-PDT mainly consisted of necrotic and late apoptotic cells. Caspase-3, caspase-8, P65, and P53 expression was upregulated in the treatment groups compared with the control group, with 5 and 10 μg/ml PSD-007 therapies, whereas no significant alteration in Bcl-2 and Bcl-x was found. Conclusion： PDT inhibits the proliferation and in- duces the apoptosis of cancer cells MDA-MB-231 by upregulating the caspase-3, caspase-8, P53, and NF-KB pathways, indicating a new strategy for treating breast cancer in the future.

关 键 词：光动力疗法 乳腺癌 癌光啉PSD-007 凋亡 

分 类 号：R737.9[医药卫生—肿瘤]