Apoptin与顺铂联用对膀胱癌T24细胞具有协同杀伤作用  

Synergistic lethal effect of Apoptin combined with diamminedichloroplatinum on T 24 cells of bladder carcinoma

作　　者：王亚林[1] 毛丽伟[2] 赵立[1] 曲楠[1] 黄晨[1] 麦海星[1] 陈立军[1] 

机构地区：[1]军事医学科学院附属医院泌尿外科,北京100071 [2]解放军309医院肿瘤科

出　　处：《西南国防医药》2013年第1期21-24,共4页Medical Journal of National Defending Forces in Southwest China

摘　　要：目的探讨pApoptin-EGFP质粒转染与顺铂(DDP)联用对体外培养的膀胱癌T24细胞的协同杀伤作用。方法脂质体介导pApoptin-EGFP瞬时转染与梯度浓度DDP共同处理膀胱癌T24细胞,流式细胞仪检测细胞增殖变化;pApoptin-EGFP瞬时转染与10μg/ml的DDP处理T24细胞,Annexin V法检测凋亡变化;金氏法分析两种抗肿瘤方式的协同作用。结果 DDP、pEGFP-N1+DDP和pApoptin-EGFP+DDP 3组的IC50分别为10.61、7.9和2.4μg/ml,以金氏法计算,确认pApoptin-EGFP质粒转染与DDP处理对T24的抑制增殖效应具有协同作用,而转染空质粒与DDP干预仅仅为简单相加。同法检测DDP、pApoptin-EGFP、pApoptin-EGFP+DDP 3组的凋亡率分别为(6.96±1.32)%、(19.55±1.09)%和(32.5±1.15)%,3组凋亡率相互间均具有显著性差异(P<0.05)。按金氏法计算,确认转染pApoptin-EGFP重组质粒与10μg/ml浓度DDP处理对T24细胞具有协同的促凋亡作用。结论 Apoptin基因转染与顺铂联用对T24肿瘤抑制增殖及诱导凋亡具有协同作用。Objective To discuss the synergistic lethal effect of pApoptin - EGFP plasmid transfection combined with diamminedichloroplatinum（DDP） on the T 24 cell of bladder carcinoma cultured in vitro. Methods T 24 cells of bladder carcinoma were treated by transient transfection of liposome mediated pApoptin - EGFP combined with gradient concentrations of DDP. The flow cytometry was used to detect the changes of cell proliferation. T 24 cells were treated by transient transfection of liposome mediated pApoptin - EGFP combined with 10 μg/ml DDP. Annexin V method was used to detect the changes of the apoptosis,and JinJs method was used to analyze the synergetic effect of the two anticancer therapies. Results The IC50 of the DDP, pEGFP - N1 ＋ DDP, and pApoptin - EGFP ＋ DDP was 10.61,7.9, and 2.4 μg/ml, respectively. The calculation by the Jin^s method identified that there was synergistic effect of the treatment by pApoptin - EGFP plasmid transfection combined with DDP on the proliferation inhibition of the T 24 cells, but the transfection and the interfere by DDP are only simple addition. The apoptotic rates of the above groups detected by the same method were（6.96 ± 1.32 ） %, （ 19.55 ± 1.09 ） %, and （ 32.5 ±1.15 ） %, respectively, and there were significant differences among the three groups （ P 〈 0.05 ）. The calculation by the Jin＇s method identified that there was synergistic apoptotic effect of the treatment by the transfection of pApoptin - EGFP recombinant plasmid and DDP with the concentration of 10 μg/ml on the T 24 cells. Conclusion Combination of Apoptin plasmid transfection and DDP has synergistic effect on the inhibition of tumor proliferation and apoptisis induction of the T 24 cells.

关 键 词：APOPTIN 顺铂 膀胱癌 协同作用 

分 类 号：R737.14[医药卫生—肿瘤]