结肠癌转移相关基因1在结肠癌组织中的表达及其临床意义  

Expression of MACC-1 in human colon cancer and its clinical significance

作　　者：王珂[1] 蒋谦[1] 俞军[2] 

机构地区：[1]南京江苏省肿瘤医院普外科,210009 [2]江苏省肿瘤医院科研科,210009

出　　处：《临床肿瘤学杂志》2012年第12期1081-1084,共4页Chinese Clinical Oncology

基　　金：江苏省卫生厅课题资助项目(H201222)

摘　　要：目的探讨结肠癌组织中结肠癌转移相关基因1(MACC-1)的表达与结肠癌临床病理特征的关系。方法采用实时荧光定量PCR和免疫印迹法(Western blotting)检测48例结肠癌组织及其相应癌旁组织中MACC-1 mRNA和蛋白的表达水平。结果结肠癌组织中MACC-1 mRNA相对表达量为(8.52±2.39)×10-4,明显高于癌旁组织中的(1.47±1.24)×10-5,差异有统计学意义(P<0.05)。结肠癌组织中MACC-1蛋白表达的灰度值为7.73±0.03,高于癌旁组织的1.02±0.01(P<0.05)。结肠癌患者组织中MACC-1 mRNA和蛋白的表达与肿瘤的分化程度、浸润深度、TNM分期显著相关(P<0.05),而与年龄、性别无关(P>0.05)。结论 MACC-1表达的检测对结肠癌的诊断具有一定的临床意义,且MACC-1的表达水平与结肠癌的浸润和转移密切相关,可作为判断结肠癌转移的肿瘤标志物之一。Objective To detect the expression of MACC-1 mRNA and protein in colon carcinoma and investigate the relation- ship with clinicopathologic characteristics. Methods The expressions of MACC-1 in 48 colon caner tissues and corresponding adjacent normal tissues were detected by real-time quantitative reverse transcription polymerase chain reaction （QT-PCR） and Western blotting. Results The expression level of MACC-1 mRNA and protein in colon cancer tissues was higher than those in adjacent normal tissues. The relative quantitative expression of MACC-I mRNA in colon cancer tissues was （8. 52 ± 2. 39） × 10-4, which significantly higher than （ 1.47 ± 1.24） ×10 -5 of adjacent normal tissues（P 〈0. 05）. The expression of MACC-1 protein in colon cancer tissues was 7.73 ± 0. 03, which higher than 1.02 ± 0. 01 of adjacent normal tissues （ P 〈 0. 05 ）. The expression of MACC-1 mRNA and protein were also positively correlated with histologic types, infiltration depth and TNM stage （ P 〈 0. 05 ）. No correlation was found in age or sex （ P 〉 0. 05）. Conclusion MACC-1 may promote the growth and metastasis of colon cancer. It will be a prognostic markers for colon cancer.

关 键 词：结肠癌 结肠癌转移相关基因1 实时荧光定量PCR 免疫印迹法 

分 类 号：R735.3[医药卫生—肿瘤]