鱼毒性藻类及其溶血活性的三维荧光光谱识别研究  

Identification of Ichthyotoxic Microalgae Species and Its Hemolytic Activity by Three-Dimensional Fluorescence Spectra

作　　者：桓清柳[1] 黄翔[1] 吴霓[1] 江涛[1] 江天久[1] 

机构地区：[1]暨南大学赤潮与海洋生物学研究中心,广东广州510632

出　　处：《光谱学与光谱分析》2013年第2期399-403,共5页Spectroscopy and Spectral Analysis

基　　金：国家重点基础研究发展规划(973)项目(2010CB428702);国家自然科学基金项目(41276091;21612401)资助

摘　　要：利用小波分析和Fisher判别等方法,研究了典型鱼毒性藻类米氏凯伦藻(karenia mikimotoi)、海洋卡盾藻(chattonella marina)及卵圆卡盾藻(chattonella ovata)在不同铁浓度培养条件下各生长期的溶血毒素与三维荧光特征光谱的关系。通过Coif2小波分析发现,鱼毒性藻类与非鱼毒性藻类的荧光特征谱差异主要集中在第1～10个数据点(波长λem=650～680nm)和35～47个数据点(波长λem=725～750nm;λex=400～425nm)的荧光强度变化。利用该波段的藻类荧光强度及其对应的溶血活性值进行Fisher判别函数分析,对鱼毒性藻类的正确判别率为91.7%,非鱼毒性藻类的正确判别率高达100%,对具中等溶血活性(≥10HU,<20HU)藻类的正确判别率为70%,而对低活性(<10HU)和高活性(≥20HU)藻类的正确判别率均达80%以上。To identify ichthyotoxic microalgae species and its hemolytic activity by three-dimensional fluorescence spectra, the re-lationship between hemolytic activity and the three-dimensional fluorescence of typical ichthyotoxic algae （Karenia mikimotoi, Chattonella marina, Chattonella ovata） cultured in different Fe^2＋ concentrations was investigated with methods of Coif2 wavelet and Fisher discriminant analysis. The results showed that the differences of three-dimensional fluorescence spectra between ich-thyotoxic algae and non-ichthyotoxic algae were within the 1 to 10 data points （wavelength λem=650～680 nm） and 35 to 47 data points （wavelength λem=725～750nm, λex=400～425 nm）. Fisher discriminant analysis showed that the correct discrimination ratios of ichthyotoxic algae and non-ichthyotoxic species are 91.7% and 100%, respectively, and the ratio of algae with medium hemolytic activity （≥10 HU,〈20 HU） was 70%and that of algae with low hemolytic activity （〈10 HU） and high hemolytic activity （≥20HU） are both of above 80%.

关 键 词：鱼毒性藻类 溶血毒素 三维荧光 

分 类 号：X55[环境科学与工程—环境工程]