农杆菌介导蝴蝶兰的遗传转化研究  

Study on Agrobacterium tumefaciens-mediated transformation of Phalaenopsis

作　　者：崔波[1,2] 蒋素华[1] 牛苏燕[2] 袁秀云[1] 马杰[1] 叶永忠[2] 

机构地区：[1]郑州师范学院,河南郑州450044 [2]河南农业大学生命科学学院,河南郑州450002

出　　处：《河南农业大学学报》2012年第6期642-645,650,共5页Journal of Henan Agricultural University

基　　金：河南省科技攻关项目(092102110128)

摘　　要：采用根癌农杆菌介导法,将ACC合成酶反义基因导入蝴蝶兰原球茎中,比较不同PPT筛选压力、Mer浓度和侵染时间等对蝴蝶兰转化的影响,优化蝴蝶兰的遗传转化体系.结果表明,在OD600值为0.2～0.3的农杆菌菌液中侵染120 min后,放入含有20 mg.L-1Mer和2.0 mg.L-1PPT的筛选培养基中的原球茎,遗传转化效率最高.ACC synzyme gene was transformed into the PLBs of Phalaenopsis by using the agrobacteri- um mediated technique, screening PPT＇ s resistant pressure, and the meropenem concentration and im- mersed time on the transformation efficiency of Phalaenopsis PLBs were compared. The screening effi- cient genetic transformation system was improved. The results showed that after an Agrobacterium tume- faciens suspension （OD600 = 0.2 -0.3） was infected for 120min, the successfully infected PLBs using the screening medium which contained 20 mg. L-1 meropenem and 2.0 mg . L-1 PPT were deter- mined.

关 键 词：蝴蝶兰 ACC合成酶基因 遗传转化 

分 类 号：Q785[生物学—分子生物学]