流式细胞术检测多发性骨髓瘤免疫表型的研究  

Study on immunophenotyping specificities in multiple myeloma detected by flow cytometry

作　　者：刘越坚[1] 张婵[2] 郭莉[1] 邱阳[1] 单路娟[1] 郭慧淑[1] 

机构地区：[1]大连医科大学附属第一医院中心实验室,辽宁大连116011 [2]辽宁省大连市妇产医院检验科,辽宁大连116033

出　　处：《中国现代医学杂志》2012年第32期42-46,共5页China Journal of Modern Medicine

基　　金：国家自然科学基金(No:30800382)

摘　　要：目的探讨多发性骨髓瘤(MM)细胞的免疫表型特征,为临床提供早期诊断、治疗及预后判断的依据。方法采用多色流式细胞术,通过CD45/SSC设门的方法。分析118例MM骨髓标本的免疫表型,并选取15例正常人浆细胞作为对照组。结果 118例MM患者中CD138、CD38、CD117、CD56、CD33、CD13、CD9、Lambda、Kappa、HLA-DR的阳性率依次为91.53%、94.92%、44.07%、72.03%、25.42%、17.80%、29.66%、39.83%、30.51%和62.71%,其他抗体阳性率极低或均不表达。结论流式细胞术免疫分型是检测MM细胞的有效方法,MM细胞的免疫分型有助于MM的准确诊断及预后监测。【Objectives】 To explore the significance of immunophenotyping specificities in multiple myeloma（MM） and provide a evidence that can improve earlier diagnosis,therapy and prognosis.【Methods】 Multi-color flow cytometry and CD45/SSC gating were used to have immunophenotyping of patients＇ bone marrow in 118 cases of multiple myeloma（MM） and 15 cases of normal plasma cells were select-ed as control at the same time.【Results】 The positive rates of CD138,CD38,CD117,CD56,CD33,CD13,CD9,LAMBDA,KAPPA,HLA-DR were 91.53%,94.92%,44.07%,72.03%,25.42%,17.80%,29.66%,39.83%,30.51%,62.71%.The expression of other antigens was low or negative.【Conclusion】 Flow cytometry is the utility method to detect multiple myeloma（MM）.The immunophenotyping analysis of multiple myeloma（MM） is helpful to diagnosis and prognosis.

关 键 词：多发性骨髓瘤 免疫表型 流式细胞术 

分 类 号：R733.3[医药卫生—肿瘤]