抗癌iRNA诱生干扰素抗癌细胞增殖效应  

STUDY ON THE EFFECT OF INTERFERON INDUCED BY ANTICANCEROUS iRNA ON THE PROLIFERATION OF CANCER CELLS

作　　者：王维忠[1] 任政华[1] 高玲[1] 李树芳[2] 侯立侠 王雪[2] 

机构地区：[1]白求恩医科大学第三临床医学院中心实验室 [2]长春市中心医院急诊科

出　　处：《白求恩医科大学学报》1991年第5期17-19,共3页Journal of Norman Bethune University of Medical Science

摘　　要：用抗癌免疫核糖核酸(iRNA)在人体内诱生IFN—α,并经提纯获得高活性IFN—α,用于癌细胞的抑制试验,并将IFN-α标记为^(125)I—IFN—α,检测癌细胞表面IFN—α受体。结果表明,提纯的IFN—α对胃癌7901细胞、Hep—2细胞、Hela细胞、L929等细胞于37℃4h孵育后,细胞膜上有^(125)I—IFN—α吸附,并与IFN—α有竞争性抑制,表现在cpm数值的增高或降低。The IFN-α with high activity induced in human by anti - cancerous iRNA was used in cancer cell inhibitory test and labelled as 125I -IFN-α to detect the IFN -α recepor on the cancer cell surface. The results showed that 125I-IFN -α can be absorbed to the membrane of gastric cancer 7901 cells, Hep-2 cells, Hela cells and L929 cells after incubation at 37℃ for 4h had a competitive inhibitory effect on cancer cells expressed as the increase and decrease of cpm values.

关 键 词：免疫核糖核酸 干扰素 癌细胞增殖 

分 类 号：R73－36[医药卫生—肿瘤]