酶-超声联合提取土人参多糖的工艺研究  

Study on technique of enzyme-ultrasonic extraction of polysaccharides from Talinum crassifolium

作　　者：黄礼德[1] 郭立强[1] 颜祖弟[1] 文全泰[1] 潘廷啟[1] 吕坤[1] 黄锁义[1] 

机构地区：[1]右江民族医学院,百色533000

出　　处：《国际生物医学工程杂志》2012年第6期353-356,共4页International Journal of Biomedical Engineering

基　　金：2013年度自治区级大学生创新训练计划立项资助项目（QJCX201338）

摘　　要：目的比较研究土人参多糖的酶法提取、超声辅助提取及2种方法联合提取的优劣。方法采用正交试验优化土人参多糖的酶法提取及超声提取方案，并研究2种方案的结合效果。结果3种提取工艺中，酶法提取多糖得率平均为17．45％，超声提取多糖得率平均为20．01％，酶-超声联合提取多糖得率平均为20．74％。结论酶-超声联合提取土人参多糖效果省时且高效。Objective To comparatively study the advantage and disadvantage of different polysaecharide extraction methods of enzymatic extraction, ultrasonic extraction and a combination of these two processes. Methods The enzymatic extraction and ultrasonic extraction were first optimized by orthogonal design and then compared with the effect of combination. Results From the results of three extraction techniques, enzymatic extraction of Talinum crassifolium polysaccharide＇s average extraction rate was 17.45%, ultrasonic extraction was 20.01%, enzyme-ultrasonic extraction was 20.74%. Conclusion The combined optimum preparative procedure of Talinum crassifolium is simple, quickly and high efficient.

关 键 词：土人参 多糖 纤维素酶 超声提取 正交设计 联合 

分 类 号：R284.2[医药卫生—中药学]