对氯甲基苯甲酸链接的莱克多巴胺人工抗原的合成与鉴定  

Synthesis and Characterization of Ractopamine Antigen Linked by 4-Methylbenzoic Acid

作　　者：陈臣[1] 罗顺菁[1] 刘成梅[1] 邹常春[2] 汪志宇[1] 王文飞[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047 [2]南昌大学资产管理处,江西南昌330031

出　　处：《食品科学》2013年第1期180-184,共5页Food Science

基　　金：江西省科技支撑计划项目(2010BNA09500)

摘　　要：采用对氯甲基苯甲酸(CBA)为链接臂,用混合酸酐法将莱克多巴胺(RAC)与牛血清白蛋白(BSA)偶联制备人工抗原(RAC-CBA-BSA),通过紫外、红外、电泳鉴定抗原合成成功;再以卵清蛋白(OVA)为载体蛋白合成包被抗原(RAC-CBA-OVA),测定其与抗体的亲和力和抑制率。间接竞争ELISA结果以对CBA为链接臂合成的包被抗原对应的抗体滴度为7047.3、IC50为17.05ng/mL,结果表明,CBA可以用于RAC人工抗原的合成,使用RAC-CBA-OVA作为包被抗原可以提高ELISA检测灵敏度。The mixed anhydride method was used to prepare artificial antigen through the conjugation between ractopamine （RAC） and bovine serum albumin （BSA） in the presence of 4-chloromethyl benzoic acid （CBA）. The prepared artificial antigen was identified by IR, UV and SDS-PAGE. RAC-CBA was also conjugated with OVA to form a coating antigen. The affinity and inhibitory activity of the coating antigen were measured. This study suggests that CBA can be used to prepare artificial RAC antigens. Meanwhile, CBA conjugated with RAC to form coating agent improves the sensitivity of ELISA.

关 键 词：莱克多巴胺 对氯甲基苯甲酸 ELISA灵敏度 人工抗原 

分 类 号：S859.84[农业科学—临床兽医学]