乳腺癌转移相关通路的基因集富集分析  

Gene set enrichment analysis of breast cancer metastasis related pathways

作　　者：余海浪[1] 刘安玲[1] 周珏宇[1] 孟伟[1] 郑文岭[2] 马文丽[1] 

机构地区：[1]南方医科大学基因工程研究所,广州510515 [2]华南基因组研究中心

出　　处：《山东医药》2013年第1期5-8,共4页Shandong Medical Journal

基　　金：广东省医学科研基金资助项目(B2012203)

摘　　要：目的探讨与乳腺癌转移相关的生物信号通路及因素。方法以乳腺癌转移相关的3组基因芯片表达谱数据为研究材料,登录号分别为GSE2034、GSE2603以及GSE12276。将乳腺癌样本分为原发癌和转移癌两组,原始数据经RMAExpress软件进行质量检验和标准化,采用GSEA通路富集工具对乳腺癌转移相关通路进行分析。结果有20条生物信号通路与乳腺癌的转移有着密切关系,其中基因集上调的有17个,下调的有3个,主要涉及细胞周期与增殖、代谢途径、血管生成、迁移、免疫系统等信号通路。结论乳腺癌转移除与肿瘤细胞的活力和增殖能力提高有关外,还与肿瘤细胞的迁移和运动能力进一步增强及机体免疫系统损害有关。Objective To investigate pathways involved in metastasis of breast cancer by use of gene expression data sets. Methods Three gene expression profiling datasets related to the breast cancer metastasis （accession number GSE2034, GSE2603 and GSE12276 respectively） were downloaded from gene expression omnibus （GEO）, which were used in this study. Breast cancer samples were separated into two groups： primary tumors and metastatic tumors. Expres- sion value of samples was obtained after raw profile data were normalized and quality control by RMAExpress software. Breast cancer metastasis related pathways were analyzed by the GSEA pathway enrichment tool. Results A total of 20 bio- logical pathways associated with breast cancer metastasis were identified, of which 17 upregulated and 3 downregulated, mainly including cell cycle and proliferation, cell adhesion, cell migration, angiogenesis, immune system and so on. Con- clusions In addition the improvement of tumor cell viability and proliferation, breast cancer metastasis is also associated with the increasement of tumor cell migration and movement and the damage of immune system.

关 键 词：乳腺肿瘤 转移 通路 基因集富集分析 

分 类 号：R737.9[医药卫生—肿瘤]