Bcl-2蛋白在宫颈癌变组织中的表达及其与人乳头瘤病毒感染的关联性  

Expression of Bcl-2 protein in cervical intraepithelial neoplasia and cervical carcinoma and its correlation with HPV16/18 infection

作　　者：黄平[1] 唐秋英 金瑛[1] 陈光元[1] 

机构地区：[1]深圳市宝安区松岗人民医院妇产科,广东深圳518105

出　　处：《海南医学》2013年第1期3-5,共3页Hainan Medical Journal

基　　金：2010年度深圳市科技计划项目(编号:201003382)

摘　　要：目的基于宫颈癌发病的病毒学原因,探讨Bcl-2基因在宫颈癌中的表达及其与人乳头瘤病毒感染的相关性。方法采用免疫组织化学法检测Bcl-2蛋白在30例宫颈上皮内瘤变(CIN)、20例宫颈癌及20例正常宫颈组织中的表达。采用PCR技术检测上述标本中HPV16/18DNA的表达。结果 Bcl-2蛋白在CIN中的表达率为40%,宫颈癌中为70%,正常宫颈中表达为5%。HPV16/18在上述组织中的表达率依次为50%、75%、5%。Bcl-2蛋白和HPV16/18DNA在正常宫颈组织、CIN及宫颈癌中的表达两两比较差异均具有统计学意义;Bcl-2蛋白在CINⅠ/Ⅱ及CINⅢ中的差异也具有统计学意义,但与宫颈癌病理类型及分化和临床分期无关。结论 Bcl-2及HPV16/18参与了宫颈癌的发生及发展,联合检查HPV16/18和Bcl-2有望为宫颈癌的临床早期诊断和治疗提供理论依据。Objective To investigate the expression of Bcl-2 protein in cervical carcinoma and cervical intraepithelial neoplasia, and to analyze the correlation between expression of Bcl-2 protein with HPV16/18 infection. Methods With immunohistochemiacal method, the expression of Bcl-2 protein was detected in 30 cases of cervical intraepithelial neoplasia （CIN）, 20 cases of cervical carcinoma and 20 cases of normal cervical tissues. Also, the HPV16/18 DNA was detected by PCR. Results The positive rates of Bcl-2 protein in the above three tissues were 40%, 70% and 5%, respectively, while the percentages of HPV16/18 infection were 50%, 75%, and 5%, respectively, both with statistically significant difference in the three tissues. The expression of Bcl-2 was related with the grade of CIN rather than clinical pathological type and clinical stage. Conclusion Bcl-2 and HPV16/18 may take part in the occurrence and development of cervical carcinoma. It would be useful to detect the Bcl-2 and HPV 16/18 in the early diagnosis and treatment for cervical carcinoma.

关 键 词：宫颈上皮内瘤变 宫颈癌 BCL-2基因 

分 类 号：R737.33[医药卫生—肿瘤]