益智仁提取物原儿茶酸对大鼠局灶性脑缺血再灌注损伤的保护作用  

Protective effect of protocatechuic acid from Alpinia oxyphylla on focal cerebral ischemic-reperfusin injury

作　　者：李智勇[1] 夏鹰[1] 陈晓东[1] 罗汉[1] 张海英[2] 

机构地区：[1]海口市人民医院神经外科,海南海口570208 [2]海南医学院人体解剖学教研室,海南海口571101

出　　处：《海南医学》2013年第2期157-159,共3页Hainan Medical Journal

基　　金：海南省自然科学基金(编号:811204);海南医学院科研培育基金(编号:HY2010-001)

摘　　要：目的应用SD大鼠局灶性脑缺血模型,研究原儿茶酸对脑缺血性再灌注损伤的保护作用。方法健康雄性成年SD大鼠36只,随机分为三组(n=12):假手术组(S组)、脑缺血再灌注组(IR组)和原儿茶酸处理组(PCA组)。采用大脑中动脉阻断法制备大鼠局灶性脑缺血再灌注模型。大鼠腹腔注射PCA,25mg/kg、1次/d、连续7d。PCA组于预处理结束后24h,制备局灶性脑缺血再灌注模型。再灌注24h后,行神经功能评分,随后处死大鼠,取脑组织,测定脑梗死体积百分比,采用PT-PCR法及Western blot测定缺血半暗带NF-κB-p65及Notch1的表达。结果与IR组比较,PCA组脑梗死体积百分比降低,神经功能评分升高,脑组织缺血区半暗带NF-κB-p65及Notch1表达下调(P<0.05)。结论 PCA预处理可能通过抑制脑组织NF-κB-p65及Notch1的表达、减轻了大鼠局灶性脑缺血再灌注的损伤。Objective To study the protective effect of protocatechuic acid （PCA） on focal cerebral isch- emic-reperfusin injury. Methods Thirty-six healthy male SD rats were randomly divided into three groups, each with 12 rats： sham operation group （group S）, ischemia-reperfusion injury group （group IR） and protocatechuic acid group （group PCA）. Focal cerebral ischemia-reperfusion injury was induced by right middle cerebral artery occlusion （MCAO） followed by 24 h reperfusion. In group PCA, rats were administrated PCA intraperitoneally at 25 mg/kg once a day for 7 consecutive days before MCAO. The neurologic function was evaluated at 24 h of reperfusion. Then the rats were sacrificed, and the brains were removed for determination of infarct volume percentage, NF-κB-p65 and Notchl expressed in ischemic penumbra by PT-PCR and Western blot. Results Compared with group IR, the infarct volume percentage, NF-κB-p65 and Notchl expression were significantly lower in group PCA, while the neurologic function score was significantly higher （P〈0.05）. Conclusion PCA can reduce focal cerebral ischemia-reperfusion injury by inhibiting the expression of NF-κB-p65 and Notch in rats.

关 键 词：脑缺血性再灌注损伤 原儿茶酸 NF-κB-p65 NOTCH1 

分 类 号：R332[医药卫生—人体生理学]