杀虫双对杜氏盐藻生长和生理生化的影响  

Effects of Chemical Pesticide Dimehypo on Growth,Physiological and Biochemical Characteristics of Dunaliella salina

作　　者：蔡马[1] 姜建国[2] 贺立红[1] 余土元[1] 

机构地区：[1]仲恺农业工程学院生命科学学院,广东广州510225 [2]华南理工大学轻工与食品工程学院,广东广州510640

出　　处：《华南农业大学学报》2013年第1期67-71,共5页Journal of South China Agricultural University

基　　金：广东省科技计划项目(2009B020307013)

摘　　要：以一种具有环境耐性的单细胞绿藻杜氏盐藻Dunaliella salina作为受试生物,检测一种杀虫剂杀虫双的毒性作用,试验中检测了杀虫双对杜氏盐藻细胞生长、单细胞β胡萝卜素质量、细胞形态变化及超氧化物歧化酶(Super-oxide dismutase,SOD)和过氧化氢酶(Catalase,CAT)活性的影响.结果表明,当杀虫双质量浓度小于0.5 mg.L-1时,细胞的毒性响应与对照的变化趋势类似.当使用1.0～4.0 mg.L-1杀虫双处理盐藻,处理初期细胞生长缓慢和单细胞β胡萝卜素质量降低,一段时间后恢复.当杀虫双质量浓度大于5.0 mg.L-1,细胞生长速度和单细胞β胡萝卜素质量都会一直降低直至检测不到.杀虫双对杜氏盐藻的10 d IC50为32 mg.L-1.试验发现在低浓度下杀虫双会刺激CAT活性的增长.Dunaliella salina, a unicellular green alga with environmental tolerance, was employed as test organism to investigate the toxicity effects of a pesticide dimehypo. The influences of dimehypo on cell growth, single cell ,8-carotene level, cell morphology changes, and activities of superoxide dismutase （SOD） and catalase （CAT） were investigated. At the mass concentrations less than 0. 5 mg·L^-1 dime- hypo, cell responses were similar to control. When treated with 1.0 -4.0 mg ·L^-1 dimehypo, cell growth and single cell β-carotene levels declined at first and then revived. When mass concentrations were higher than 5.0 mg ·L^-1dimehypo, both cell growth and single cell β-carotene levels decreased until they were undetectable. The 10 d IC50 of dimehypo on D. salina was 32 mg·L^-1. Dimehypo could stimulate the increase of CAT activity at a low concentration.

关 键 词：杜氏盐藻 毒性 杀虫双 

分 类 号：Q945.78[生物学—植物学]