慢性淋巴细胞白血病免疫表型及凋亡相关蛋白分析  

Analysis on immunophenotypes and expression of apoptosis protein of chronic lymphocytic leukemia

作　　者：刘越坚[1] 郭莉[1] 邱阳[1] 单路娟[1] 郭慧淑[1] 张婵[2] 

机构地区：[1]大连医科大学附属第一医院中心实验室,辽宁大连116011 [2]大连市妇产医院检验科,辽宁大连116033

出　　处：《国际检验医学杂志》2013年第1期25-26,共2页International Journal of Laboratory Medicine

摘　　要：目的探讨慢性淋巴细胞白血病(CLL)免疫表型和凋亡相关蛋白表达特征及其临床意义。方法应用CD45/SSC设门方案,通过三色流式细胞术对150例CLL患者、105例急性B淋巴细胞白血病(B-ALL)患者骨髓或外周血进行免疫分型。结果 150例CLL患者均高表达CD19;HLA-DR、CD20、CD5、CD22、CD38、CD23阳性率依次为96.67%、95.33%、84.67%、86.67%、40.67%、60.66%;CD10、cCD79a、CD3、CD25、CD34、ZAP70、FMC7阳性率较低,依次为3.33%、15.33%、4.67%、1.33%、3.33%、11.43%、8.67%。其他抗体均无表达;与B-ALL患者相比,CLL患者CD5、CD20阳性率升高(P<0.05),CD10、CD34、CD22、Ccd79a阳性率降低(P<0.05),CD19、HLA-DR表达率无统计学差异(P>0.05)。CLL患者凋亡相关蛋白Bcl-2、Bax阳性率分别为74.67%和7.33%。结论 CD5、CD20、CD19是CLL特异性免疫表型,ZAP70、CD38可作为预后判断指标,CLL细胞Bcl-2高表达、Bax低表达,在疾病发生、发展中意义重大。Objective To investigate the characteristics of immunophenotyping and expression of apoptosis protein in chronic lymphocytic leukemia（CLL） and related clinical significance. Methods CD45/SSC gating and three colors flow cytometry were used to analyze immunophenotyping and expression of apoptosis protein in 150 patients with CLL and 105 patients with acute B- lymphoblastic leukemia（B-AI.L）. Results CD19 was highly expressed in CLL＇patients. The positive rates of HLA-DR,CD20,CDS, CD22,CD38,CD23 were 96.67%,95.33%,84.67%0,86.67%,40.67% and 60. 66%,those of CD10,cCD79a,CD3,CD25,CD34, ZAP70 and FMC7 were low, which were 3.3＂3 %, 15.33 %, 4.67 %, 1.33 %, 3.33 %, 11.43 % and 8.67 %. The other antigens were negative. Compared with B-ALL patients,the positive rates of CD5 and CD20 were significantly higher in CLL patients and those of CD10, CD34, CD22 and cCD79a were significantly lower（P〈0.05）, but there were no significant differences of CD19 and HLA-DR between the two groups（P〉0.05）. The positive rates of Bel-2 and Bax were 74. 67% and 7. 33%. Conclusion CD5,CD20 and CD19 could be specific immunophenotic markers in CLL. ZAP70 and CD38 could be used as prognosis markers in CLL. High level of Bcl-2 and low level of Bax in CLL might play a pivotal role in pathogenesis and progression of CLL.

关 键 词：传染性单核细胞增多症 TH17细胞 外周血单个核细胞 

分 类 号：R733.7[医药卫生—肿瘤]