人参皂甙Rg3对肺癌荷瘤小鼠免疫功能影响的研究  

Study on the Effect of Ginsenoside Rg3 on Immune Function of Tumor-Bearing Mice

作　　者：王家晓[1] 张玉梅[1] 

机构地区：[1]广西中医药大学第一附属医院肿瘤血液科,南宁市530023

出　　处：《广西医学》2013年第1期13-15,共3页Guangxi Medical Journal

基　　金：广西自然科学基金项目(2010GXNSFA013213);广西青年基金项目(2011GXNSFB018104);广西中医药大学重点课题(2D2009003)

摘　　要：目的探讨人参皂甙Rg3对肺癌荷瘤小鼠免疫功能的影响。方法将48只C57BL/6肺癌荷瘤小鼠随机分为4组:人参皂甙Rg3高剂量组(15 mg/kg)、人参皂甙Rg3低剂量组(5 mg/kg)、顺铂(DDP)组(DDP0.5 mg/kg)和生理盐水组,检测各组给药后荷瘤小鼠的脾自然杀伤细胞(NK)、淋巴因子激活的杀伤细胞(LAK)、细胞毒性T细胞(CTL)活性及血清肿瘤坏死因子(TNF)水平。以正常小鼠12只作为对照组。结果 Rg3低剂量组、Rg3高剂量组、DDP组生存素阳性率明显低于生理盐水组(P<0.05);Rg3低剂量组、Rg3高剂量组瘤体质量小于生理盐水组(P<0.05),但大于DDP组(P<0.01);生理盐水组NK、LAK和CTL活性及血清TNF水平均较正常对照组显著下降(P<0.05);DDP组NK、LAK和CTL活性及血清TNF水平较生理盐水组低(P<0.05);Rg3低剂量组和Rg3高剂量组NK、LAK和CTL活性及血清TNF水平较生理盐水组、DDP组明显提高(P<0.05),但仍低于正常小鼠组(P<0.05);Rg3低剂量组NK、LAK和CTL活性及血清TNF水平与Rg3高剂量组比较,差异无统计学意义(P>0.05)。结论人参皂甙Rg3可明显诱导提高荷瘤小鼠特异性抗肿瘤免疫反应。Objective To investigate the effect of Ginsenoside Rg3 on the immune function of tumor-bearing mice with lung cancer. Methods Forty-eight tumor-bearing mice with C57BL/6 lung cancer were randomly divided into four groups,high-dose Ginsenoside Rg3 group（Rg3,15 mg/kg）, low-dose Ginsenoside Rg3 group（Rg3,5 mg/kg）, cisplatin （DDP） group（DDP,0.5 mg/kg） and saline group. The activities of splenic natural killer（NK） cells,lymphokine activated killer（LAK） cells,cytotoxic T lymphocyte（CTL） ,and serum tumor necrosis factor（TNF） level were detected in each group after the administration of tumor-bearing mice was performed. Twelve normal mice as control group. Results The positive rates of Surv-ivin of low-dose Rg3 group,high-dose Rg3 group,DDP group were significantly lower than that of saline group （P 〈0.05） ;Tumor mass of low-dose Rg3 group,high-dose Rg3 group was less than that of saline group （P 〈0.05）,but greater than that of DDP group（P 〈0.01 ） ;The activities of NK,LAK and CTL,and serum TNF level decreased significantly in saline group compared with the normal controls（P 〈0.05）;The activities of NK,LAK and CTL,and serum TNF level of DDP group were lower than those of saline group （ P 〈 0.05 ）, which increased significantly in low-dose Rg3 group and high-dose Rg3 group compared with those of saline group, DDP group （ P 〈 0.05 ）, but lower than the normal controls （P 〈 0.05） ;There was no significant difference in the activities of NK, LAK and CTL, and serum TNF level between low-dose P~,3 group and high-dose Rg3 group （P 〉 0. 05 ）. Condusion Ginsenoside Rg3 can improve specific anti-tumor immune response in tumor-bearing mice significantly.

关 键 词：肺癌 人参皂甙RG3 免疫功能 小鼠 

分 类 号：R734.2[医药卫生—肿瘤]