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作 者:郭冬生[1] 彭小兰[1] 王兴平[1] 龚群辉[1]
机构地区:[1]湖南文理学院生命科学院,动物学湖南省高校重点实验室,湖南常德415000
出 处:《农业现代化研究》2013年第1期122-127,共6页Research of Agricultural Modernization
基 金:湖南省自然科学基金(编号:12JJ9007);湖南省教育厅优秀青年项目(编号:11B088);“动物学”湖南省“十二·五”重点建设学科(编号:湘教发[2011]76)
摘 要:以装有永久性瘤胃瘘管的黄牛作为瘤胃液的供体动物,应用短期人工瘤胃发酵技术和纯化学分析方法,分别测定17种饲料样品的CNCPS组分、体外培养发酵24h的uCP、发酵液的NH3-N浓度和pH值,研究饲料CNCPS组分与uCP的相关性。结果表明,uCP与饲料中的NDF、ADF和CF含量呈负相关关系;uCP与饲料中的CP、EE和NPN含量呈正相关关系;uCP与CNCPS组分中的PA、PB2、PB3、PC呈极显著的正相关关系,与CC、CB2、CA呈极显著的负相关关系;与uCP关系最显著的CNCPS指标为PB3和PB2。The objective of this experiment was to study the correlation between the utilizable crude protein (uCP) and CNCPS components of the seventeen conventional ruminant feedstuffs through chemical analysis methods and short term artificial rumen technique. In this study, one adult cattle fitted with a perpetual large rumen cannula was used as the donor of rumen fluid, rumen fluid was collected and cultivated in vivo. The results showed that there was a positive correlation between the utilizable crude protein and CP, EE, NPN content, however, there was negative correlation between the utilizable crude protein and NDF, ADF, CF. There was significant positive correlation between the utilizable crude protein and PA, PB2, PB3 and PC. But there was significant negative correlation between the utilizable crude protein and CC, CB2 and CA. The regression formula was established as uCP ( % ) =0.209 PA-0.438 PB1+0.606 PB2+ 1.664 PB3+2.330 PC-1.219 CC-- 1.121 CB2+0.275 CB1-1.285 CA+ 111.862 ( R2 =0.904, n=17, P =0.008 〈0.01), which had preferable practical value.
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