c-jun氨基末端激酶在兔蛛网膜下腔出血后脑血管痉挛中的作用  

Effect of JNK on the cerebral vasospasm after subarachnoid hemorrhage in rabbit

作　　者：游鸿海[1] 康德智[1] 王灯亮[1] 张元隆[1] 

机构地区：[1]福建医科大学附属第一医院神经外科,福建福州350005

出　　处：《中国老年学杂志》2013年第2期334-336,共3页Chinese Journal of Gerontology

基　　金：福建省科技厅重点项目(No.2009Y0018)

摘　　要：目的观察兔蛛网膜下腔出血(SAH)后基底动脉中原癌基因c-jun氨基末端激酶(JNK)的表达规律,以探讨其与脑血管痉挛(CVS)的关系。方法将72只新西兰大白兔随机分为2组:①对照组:12只;②SAH组:60只。SAH组又随机分为注血后1、3、5、7、10 d,共5组,每组12只。采用枕大池二次注血法建立稳定的兔CVS模型,应用苏木素-伊红染色和Western印迹分别观察兔基底动脉的形态学改变和JNK的表达情况,并通过测量管径的变化来判断CVS的严重程度。结果 SAH组的基底动脉出现相应的病理学改变,管腔狭窄呈急性期收缩和迟发性痉挛双相改变;SAH组的JNK表达升高,在第7天时达到高峰(P<0.01),第10天后表达稍下降,但仍高于对照组(P<0.01)。结论在兔CVS模型的基底动脉中JNK的表达明显上调,呈现一定的时序性变化规律,与迟发性CVS的严重程度一致,在CVS的发生和发展过程中起了重要的作用。Objective To observe the expression of c-jun N-terminal kinase （JNK） in the basiliar arteries after subarachnoid hem- orrhage （SAH） in rabbits and discuss the relations between the expression of JNK and cerebral vasospasm （CVS）. Methods 72 New Zeal- and white rabbits were randomized divided into control and SAH groups （including 1,3, 5, 7 and 10 d, n = 12）. A steady model of cere- bral vasospasm after SAH in rabbits was produced by two-time blood injection of autologous arterial blood into the cisterna magna. HE stai- ning and Western blot were respectively used to observe the morphological changes and the expressions of JNK in the basilar artery. The di- ameter of each basilar arterial lumen was measured to evaluate the degree of cerebral vasospasm. Results The basilar artery in SAH groups existed corresponding pathological changes, this model showed biphasic patterns to the luminal narrowing：acute constriction and delayed vas- ospasm phase. The expressions of JNK in SAH groups were increased compared to control group, and achieved peak at 7 days after SAH （ P 〈 0. 01 ） , then decreased 10 days after SAH, but it was still significantly higher than that of control group （P 〈 0. 01 ）. Conclusions The expression of JNK is obvious increased and shows the regular change in time course, which is in proportion to the extent of delayed cere- bral vasospasm, and it is important significance in the course of occurrence and development after CVS.

关 键 词：蛛网膜下腔出血 脑血管痉挛 JNK 细胞凋亡 

分 类 号：R361.3[医药卫生—病理学]