血管瘤演变过程中过氧化物酶体增殖物激活受体-γ基因表达规律及与脂肪形成的关系  

The role of the expression of PPAR-γ gene in the adipogenesis in hemangioma evolution

作　　者：袁斯明[1] 陈荣亮[1] 陈海妮[2] 沈卫民[2] 周晓军[1] 

机构地区：[1]南京军区南京总医院病理科,南京210002 [2]南京市儿童医院整形外科

出　　处：《中华整形外科杂志》2013年第1期45-48,共4页Chinese Journal of Plastic Surgery

基　　金：国家自然科学基金资助项目（30700874）;中国博士后科学基金资助项目（20090451571）;江苏省博士后科研资助计划项目（0901062C）;南京军区南京总医院科研基金资助（2009Q038）

摘　　要：目的探讨血管瘤演变过程中，过氧化物酶体增殖物激活受体．1基因（Peroxisome proliferator activated receptors-γ，PPAR-γ）表达规律及其和脂肪形成的关系。方法以Perilipin A为标志蛋白进行常规免疫组化染色，观察各阶段血管瘤中的脂肪形成。应用荧光免疫组化染色，观察控制脂肪形成的关键转录因子PPAR-γ在血管瘤组织中的表达定位，以a-SMA和CD31作共染色。应用RT·PCR检测PPAR-γ基因在各阶段血管瘤组织中的表达。结果在血管瘤演变过程中，瘤体内脂肪组织逐渐增多，最终被纤维脂肪组织代替。PPAR-γ主要表达于微血管周围的细胞核。增生期、消退期和消退完成期血管瘤组织的PPAR-γ基因相对表达强度逐渐增高，但低于正常皮下脂肪组织。结论PPAR-γ主要表达于微血管周围细胞，提示可能与血管瘤消退期脂肪形成有关。Objective To investigate the role of the expression of PPAR-γ,gene in the adipogenesis in hemangioma evolution. Methods Routine immunohistochemistry staining of Perilipin A, the marker antigen of adipocytes, was performed to observe the adipogenesis in hemangioma. Immunofluorescencc staining of PPAR-γ,, the important transcription factor in promoting adipogenesis, was carried out to observe its location in hemangioma tissue, with the co-staining of a-SMA and CD31. And RT-PCR was used to examine the expression of PPAR-γ gene in hemangioma in different stages. Results In the evolution of hemangioma, the number of adipocytes increased continuously. And the tumor was replaced by fibrofatty tissue finally. PPAR-γ was located in the nuclei of perivascular cell in hemangioma tissue. The expression of PPAR-γ gene in hemangioma increased in the evolution of hemangioma, but still was lower than that in normal fat tissue from children. Conclusion The expression of PPAR-γ in the perivascular cells suggests that they may contribute to the adipogenesis in hemangioma involution.

关 键 词：血管瘤 脂肪形成 微血管周围细胞 过氧化物酶体增殖物激活受体-Γ 

分 类 号：R739.5[医药卫生—肿瘤]