4-氨基-2-三氟甲基维甲酸酯对乳腺癌细胞株MDA-MB-231迁移的影响及其可能机制  

Effects of its probable mechanism of a synthetic retinoid 4-amino-2-tri-fluoromethyl-phenyl ester on the migration ability of human breast cancer MDA-MB-231 cells

作　　者：王北[1] 颜蕴文[1] 周青[1] 桂淑玉[2] 汪渊[1] 

机构地区：[1]安徽医科大学分子生物学实验室、生物化学与分子生物学教研室、安徽省/省部共建教育部重要遗传病基因资源利用重点实验室,合肥230032 [2]安徽医科大学第一附属医院呼吸内科,合肥230022

出　　处：《安徽医科大学学报》2013年第2期115-119,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金资助项目(编号:81272399);安徽省自然科学基金(编号:90413116)

摘　　要：目的研究维甲酸衍生物4-氨基-2-三氟甲基维甲酸酯(ATFMPE)对乳腺癌细胞株MDA-MB-231细胞迁移的影响及可能机制。方法不同浓度维甲酸(ATRA)和ATFMPE处理乳腺癌细胞株MDA-MB-231 48 h后,用MTT法检测ATFMPE对细胞增殖的影响,划痕法检测对细胞迁移的影响,Western blot法检测相关迁移蛋白的表达。结果 AT-FMPE对乳腺癌细胞株MDA-MB-231细胞增殖具有明显的抑制作用,ATFMPE和肌球蛋白轻链激酶(MLCK)抑制剂ML-7对细胞的迁移具有明显的抑制作用,Western blot结果显示ATFMPE显著降低MLCK的表达和肌球蛋白轻链(MLC)的磷酸化。结论 ATFMPE对乳腺癌细胞株MDA-MB-231细胞的迁移具有抑制作用,可能与MLCK表达和MLC磷酸化水平下调相关。Objective To explore the effect of its probable mechanism of a synthetic retinoid 4-amino-2-tri-fluorom- ethyl-phenyl ester （ATFMPE）on the migration ability of human breast cancer MDA-MB-231 cells. Methods MTT assay was performed to measure the proliferation of MDA-MB-231 cells treated with different concentrations of AT- FMPE. Light microscope was used to observe morphologie changes. The effect of ATFMPE and ML-7, a selective in- hibitor of myosin light chain kinase （MLCK） , on the migration of MDA-MB-231 cells was analyzed by cell migra- tion test. The expression of MLCK and phosphorylation of myosin light chain （MLC） proteins was detected by West- ern blot. Results ATFMPE could inhibit the proliferation of breast cancer MDA-MB-231. ATFMPE and ML-7 could decrease the distances of migration of breast cancer MDA-MB-231 significantly. ATFMPE could inhibit the expression of MLCK and phosphorylation of MLC protein. Conclusion ATFMPE could inhibit the migration of breast cancer MDA-MB-231 cells, which may be related to the down regulation of expression of MLCK and phosphorylation of MLC protein.

关 键 词：4-氨基-2-三氟甲基维甲酸酯 乳腺癌细胞株MDA—MB-231 肌球蛋白轻链激酶 

分 类 号：R739.5[医药卫生—肿瘤] R966[医药卫生—临床医学]