美斯地浓磷脂复合物的制备及其对大鼠在体肠吸收的促进作用  

Preparation of mestinon-phospholipid complex and its promoting effect on intestinal absorption in rats

作　　者：胡霓霓[1] 杨林 滕永真[1] 黄开顺[1] 张景勍[1] 

机构地区：[1]重庆医科大学药物高校工程研究中心,重庆400016 [2]重庆市食品药品检验所,重庆401121

出　　处：《第二军医大学学报》2013年第1期53-57,共5页Academic Journal of Second Military Medical University

基　　金：教育部高等学校博士学科点专项科研基金(20095503120008)~~

摘　　要：目的研究美斯地浓磷脂复合物(MSPLC)对美斯地浓在大鼠体内肠吸收的促进作用。方法制备MSPLC,并测定其复合率、理化性质及表观油/水分配系数。选择健康SD雄性大鼠6只,分为2组,利用大鼠在体肠吸收模型,研究MSPLC及美斯地浓的吸收速率常数和有效渗透率。结果 MSPLC复合率为(84.02±1.68)%;MSPLC的DSC图谱中,美斯地浓的吸收峰消失,且相变温度比磷脂低;紫外光谱与美斯地浓的紫外光谱相似;傅里叶-红外扫描图谱与美斯地浓和磷脂的物理混合物有明显的不同。MSPLC平均粒径为(204.6±0.3)nm,zeta电位为(-25.12±0.16)mV,粒径分布基本符合正态分布;肠循环液中美斯地浓回收率与精密度符合要求;MSPLC表观油/水分配系数约为美斯地浓原料药的17倍;MSPLC的吸收速率常数和有效渗透率分别是美斯地浓的2.32倍和2.52倍。结论 MSPLC能明显改善美斯地浓在大鼠小肠的吸收。Objective To study the role of mestinon-phospholipid complex in promoting the intestinal absorption of mestinon in rats. Methods The mestinon-phospholipid complex was prepared and its composition rate, physico-chemical property and N-octanol/water partition coefficient were examined. Six healthy male rats were randomly divided into two groups. Using the rat model of intestinal absorption, we studied the intestinal absorption rate constant and effective permeability of mestinon-phospholipid complex and free mestinon. Results The composition rate of mestinon-phospholipid complex was （84.02±1.68）%. The characteristic endothermal peak of mestinon disappeared in the DSC curve of mestinon-phospholipid complex. The phase transition temperature of the complex was lower than that of phospholipids, the ultraviolet spectrum profile of the complex was similar to that of mestinon, and the infrared spectrum of the complex was significantly different from that of the physical mixture of mestinon and phospholipid. The particle size of mestinon-phospholipid complex was about （204.6±0.3） nm,and its zeta-potential was （-25.12±0.16） mV. The particle size was largely in a normal distribution. Both the coefficient of recovery and precision of mestinon in intestinal circulating fluid met the requirement. The N-octanol/water partition coefficient of mestinon-phospholipid complex was about 17 folds that of mestinon. The absorption rate constant and the effective permeability of mestinon-phospholipid complex were 2.32 and 2.52 folds those of free mestinon, respectively. Conclusion The mestinon-phospholipid complex can greatly improve the intestinal absorption of mestinon in rats.

关 键 词：美斯地浓 磷脂类 磷脂复合物 肠吸收 

分 类 号：R943.44[医药卫生—药剂学]