幼鼠中枢神经系统FOS蛋白表达升高与母婴分离相关  

Relationship between the FOS expression increase in central nervous system of developing rats and neonatal maternal separation

作　　者：林滨榕[1] 吴国媛[1] 吴斌[1] 陈素清[1] 林春[2] 

机构地区：[1]福建医科大学附属第一医院儿科,福建福州350005 [2]福建医科大学附属第一医院疼痛研究室,福建福州350005

出　　处：《基础医学与临床》2013年第2期205-210,共6页Basic and Clinical Medicine

基　　金：福建医科大学教授学术发展基金(JS08011);福建省卫生厅青年科研课题(2008-2-22)

摘　　要：目的探讨幼鼠中枢神经系统下丘脑室旁核(PVN)及前皮质扣带回(ACC)内FOS蛋白表达与母婴分离(MS)的相关性。方法按析因设计,32只SD新生大鼠分成4组,每组8只。A1B1组、A1B2组均为MS组,A1B1组在6周龄时接受结直肠扩张刺激(CRD),A1B2组在6周龄时未给予CRD;A2B1组、A2B2组均未给予MS,同为对照组,A2B1组在6周龄时接受CRD,A2B2组在6周龄时未给予CRD。A1B1、A2B1组幼鼠在接受CRD刺激后2 h和A1B2、A2B2组幼鼠一起处死。之后采用SABC免疫组化法检测不同脑区(包括PVN和ACC)内FOS蛋白免疫阳性(FLI)细胞表达情况。结果新生期MS和幼鼠在6周龄时接受CRD刺激均可使PVN内FLI细胞数显著升高(F分别为7.033、35.212,P<0.01)。也均使ACC内FLI细胞数显著升高(F分别为22.745、15.106,P<0.01);两者对FOS蛋白表达的影响无交互作用。结论幼鼠中枢神经系统(PVN和ACC)FOS蛋白表达升高与新生期MS明显相关。Objective To study the correlation between neonatal maternal separation （MS） and the FOS expression in hypothalamus paraventricular nucleus（PVN） and anterior cingulated cortex（ACC） in central nervous system in developing rats. Methods According to the factorial design, 32 SD rats were divided into four groups with 8 in each. Group AIBl and group AIB2 were MS group. Group AIB1 were imposed on CRD at 6-week age, yet group A1B2 was not imposed on CRD. Group A2B1 and group A2B2 both as the control groups. Group A2B2 was imposed on CRD at 6-week age, while group A2B2 were untreated. The rats were killed after the group Al B1 and A2B1 were treated by CRD for 2 hours while the group A1BE and A1 BE were not exposed to CRD. The semiquantity analysis of the FOS-Like Immunoreactivity（FLI） cell numbers of different sites（ including PVN and ACC ） in central nervous system were made by immuno-hisrochemical staining. Results Both neonatal MS and CRD at 6-week age could significantly increase the of FLI cell numbers, not only in PVN but also in ACC . There was no interaction between neonatal MS and infant CRD on the expression of FOS in PVN and ACC. Conclusions The increase of FOS expres-sion in PVN and ACC in central nervous system in developing rats is related to neonatal MS obviously.

关 键 词：FOS蛋白表达 幼鼠 母婴分离 下丘脑室旁核 前皮质扣带回 

分 类 号：R363[医药卫生—病理学]