腺病毒介导nm23-H1基因在Tca8113细胞中表达的实验研究  

Study on the Expression of nm23-H1 Gene in Tca8113 Cell Line Mediated by Adenovirus

作　　者：范红渠[1] 周卫兵[1] 郝鲁峰[1] 邵磊[1] 王智勇[1] 

机构地区：[1]东风汽车公司襄樊医院口腔科,湖北襄阳441004

出　　处：《口腔医学研究》2013年第1期13-16,20,共5页Journal of Oral Science Research

基　　金：湖北省襄阳市重点科技攻关计划项目(编号:2007117)

摘　　要：目的:利用人nm23-H1基因重组腺病毒转染Tca8113细胞,检测nm23-H1基因在Tca8113细胞中的表达。方法:制备人nm23-H1基因重组腺病毒并转染人舌癌Tca8113细胞系,采用Western blotting、免疫荧光法、免疫组化法和流式细胞术检测nm23-H1基因的表达。结果:Western blotting、免疫荧光法、免疫组化法和流式细胞术均检测到nm23-H1在Tca8113细胞中的表达,表达产物主要存在于细胞质中。结论:重组腺病毒载体Ad-nm23-H1成功介导了Tca8113细胞内nm23-H1基因的表达,为肿瘤的基因治疗提供了依据。Objective： To analyze the expression of nm23-H1 gene in Tca8113 cells transfected by recombinant adenovirus. Methods： Tca8113 cells were transfected with Ad-nm23-H1. The expression of nm23-H1 gene in Tca8113 cells was determined by Western blotting, immunofluor-escence assay（IFA）,immunohistochemistry assay（IHA） and flow cytometry. Results.. The expression of nm23-H1 protein was proved in Tca8113 cells by Western blotting, IFA, IHA and flow cytometry, and the expressed product mainly existed in cytoplasm. Conclusion：Ad -rim23-H1 successfully mediates the expression of nm23-H1 gene in Tca8113 cells which provided a basis for gene therapy of tumors.

关 键 词：NM23-H1 重组腺病毒 表达 

分 类 号：R739.8[医药卫生—肿瘤]