根尖终末工作宽度对根管内细菌清除效果的体外研究  

In Vitro Evaluation of the Effectiveness of Intracanal Bacterial Removal Related to Apical Terminal Working Widths

作　　者：罗玉[1] 杨健[1] 

机构地区：[1]南昌大学附属口腔医院牙体牙髓科,江西南昌330006

出　　处：《口腔医学研究》2013年第1期55-57,60,共4页Journal of Oral Science Research

基　　金：江西省教育厅科学技术研究项目(编号:GJJ11342)

摘　　要：目的:比较不同的根尖预备终末工作宽度对根管内细菌清除效果的影响。方法:将已感染粪肠球菌的48颗离体上颌磨牙的远中颊侧根管标本随机分为6组,实验组分别预备根管至25#(A组)、30#(B组)、35#(C组)、40#(D组);对照组E、F组不进行机械预备而只进行冲洗。采用ProTaper机用镍钛锉和不锈钢K锉冠向下法预备根管。A、B、C、D、E组用1%次氯酸钠和17%EDTA冲洗根管,F组仅用生理盐水冲洗。根管预备前和预备后分别取样作细菌学培养计数,并做统计学分析。结果:预备冲洗后6组根管内的粪肠球菌量均显著下降(P<0.05),其中D组下降最明显。A、B组间差异无统计学意义,其余各组间比较均有显著性差异(P<0.05)。在实验组A、B、C、D组中,根管预备后阴性培养的百分率分别为0%、12.5%、37.5%和75%;对照组E、F组中均未获得阴性培养。结论:增大根尖预备终末工作宽度能提高根管内细菌清除的效果。但上颌磨牙远中颊侧根管预备至40#配合抗菌性冲洗液,尚不能完全清除根管内粪肠球菌。Objective： To compare and evaluate the effect of intracanal bacterial removal related to different apical terminal working widths of apical preparation. Methods： Forty-eight distobuccal root canals of maxillary molars teeth inoculated with Enterococcus faecalis were randomly and evenly divided into six groups, which underwent different apical preparation sizes： group A was 25# , group B was 30# , group C was 35# , group D was40# , and group E and F were the control groups with root cananl irrigation and no mechanical instrumentation. Root canals were prepared with ProTaper rotary instruments and stainless steel K files using a crown-down technique. Group A,B,C,D,E were irrigated with 1% sodium hypochlorite and 17% EDTA;Group F was irrigated with 0.9% saline water. Microbiological samples were taken from the root canals before preparation and after preparation. The colony forming units （CFUs） were counted and analyzed. Results： The numbers of Enterococcus faecalis in root canals in each of the six groups were effectively reduced （P〈0. 05）, and group D showed maximum reduction in CFUs. There was no statistically significant diference between group A and group B, while differences between other groups were significant （P〈0.05）. In groups A,B,C and D, percentages of postpreparation negative cultures were 0%, 12.5% ,37.5% and 75%, respectively. In the control groups E and F, none of the specimens showed negative culture. Conclusion.. The effect of reducing intracanal bacterial was improved with progressively larger apical preparation size. But a complete elimination of the intracanal bacteria was not achieved when distobuccal root canals of maxillary molars teeth were prepared to apical size 40# with antibacterial irrigation.

关 键 词：工作宽度 根尖预备 粪肠球菌 

分 类 号：R781.05[医药卫生—口腔医学]