Dicer表达与肾透明细胞癌发生及转移的关系  

Dicer expression in relation to tumorigenesis and metastasis of clear cell renal cell carcinoma

作　　者：范阳[1] 黄庆波[1] 高宇[1] 艾青[1] 倪栋[1] 陈伟浩[1] 马鑫[1] 张旭[1] 

机构地区：[1]中国人民解放军总医院泌尿外科,北京100853

出　　处：《临床泌尿外科杂志》2013年第1期6-9,共4页Journal of Clinical Urology

基　　金：国家自然科学基金资助项目(编号30972982)

摘　　要：目的:探讨Dicer在肾透明细胞癌发生及转移中的作用。方法:选取正常肾小管上皮细胞株HKC、非转移性肾透明细胞癌细胞株769-P、转移性肾透明细胞癌细胞株Caki-1以及36例肾透明细胞癌手术标本(其中11例已发生远处转移)和相应癌旁正常肾组织,应用实时定量PCR和Western blot方法检测Dicer在肾透明细胞癌细胞株和组织中mRNA和蛋白的表达情况,并分析Dicer的mRNA水平与临床病理资料的关系。结果:和正常肾小管上皮细胞株HKC相比,Dicer的mRNA水平在肾透明细胞癌细胞株769-P和Caki-1中均降低(P<0.001),而转移性肾透明细胞癌细胞株Caki-1比非转移性肾透明细胞癌细胞株769-P表达水平更低(P<0.001);和癌旁正常肾组织相比,Dicer的mRNA水平在肾透明细胞癌手术标本中明显降低(P<0.001),且已发生远处转移的肾癌标本比未发生远处转移的肾癌标本表达水平更低(P=0.04);Dicer在细胞株和组织中的蛋白水平的变化与mRNA水平的变化一致(P<0.001);Dicer的mRNA水平在不同年龄、性别、组织学分级、肿瘤大小及T分期组间无统计学差异(P>0.05)。结论:Dicer表达降低可能在肾透明细胞癌的肿瘤发生中发挥作用,且其表达的进一步下降可能与肾透明细胞癌的远处转移有关。Objective：To explore the role of Dicer in tumorigenesis and metastasis of clear cell renal cell carci-noma （ccRCC）. Method：The expression of Dicer in mRNA and protein levels were detected in human kidney tubule epithelial cell line HKC, non-metastatic ccRCC cell line 769-P, metastatic ccRCC cell line Caki-1, and 36 cases of ccRCC surgical specimens（including 11 cases with distant metastasis）and their corresponding adjacent normal renal tissues by real-time PCR and Western blot respectively, analyzing the relationship between the mRNA levels of Dicer and clinicopathological variables. Result：Compared to human kidney tubule epithelial cell line HKC, the mR- NA levels of Dicer were significantly down regulated in ccRCC cell lines of 769-P and Caki 1 （ P 〈0. 001）, with the metastatic ccRCC cell line Caki-1 even lower（ P 〈0. 001）. Meanwhile, the mRNA levels of Dicer were signifi- cantly down-regulated in ccRCC surgical specimens compared to adjacent normal renal tissues （ P〈0.001）, with the metastatic ones further reduced （ P =0. 04）. The alteration of the protein levels in ccRCC cell lines and tissues were consistent with that of the mRNA levels （ P〈0. 001）. The mRNA levels of Dicer were not significantly dif-ferent among different groups, in terms of age, sex, histological staging, tumor size and T staging （ P〉0.05）.Conclusion： Reduced expression of Dicer may play a role in the tumorigenesis of ccRCC, and expression of a furtherdecline may relate to distant metastasis of ccRCC.

关 键 词：肾透明细胞癌 DICER 转移 

分 类 号：R737.11[医药卫生—肿瘤]