胃癌MKN45细胞培养液上清对腹膜间皮细胞损伤的实验研究  

Experimental study on destruction of gastric cancer MKN45 cells culture medium to peritoneum mesothelial cells

作　　者：那迪[1] 刘福囝[1] 姜成钢[1] 徐昊[1] 王振宁[1] 徐惠绵[1] 

机构地区：[1]中国医科大学附属第一医院肿瘤外科,辽宁沈阳110001

出　　处：《现代肿瘤医学》2013年第2期235-238,共4页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:81071956)

摘　　要：目的:建立胃癌腹膜转移早期间皮细胞的损伤模型,探讨胃癌腹膜转移早期癌细胞对间皮细胞的损伤与细胞凋亡的联系,以及凋亡程度与相关蛋白表达。方法:将人胃癌细胞培养液上清,与人腹膜间皮细胞系HMrSV5共培养。观察间皮细胞形态、增殖改变,透射电镜观察细胞凋亡,流式细胞仪和荧光染色判断凋亡比例。免疫印迹法检测凋亡相关蛋白Bcl-2和Bax表达情况。结果:接触胃癌培养液上清24h后,间皮细胞大片脱落并出现典型凋亡表现。胃癌细胞的分泌物明显抑制间皮细胞的生长。胃癌细胞培养液上清可诱导间皮细胞凋亡、抑制细胞增殖,呈程度-时间依赖性地抑制间皮细胞抗凋亡蛋白Bcl-2的表达;并能增加促凋亡蛋白Bax表达。结论:胃癌细胞在腹膜转移早期可以通过其分泌物诱导间皮细胞损伤、凋亡,凋亡机制与Bcl-2和Bax的失调有关。提示保护间皮细胞,抗间皮细胞凋亡在胃癌腹膜转移治疗中可能有一定应用价值。Objective：To examine the mechanism by which the gastric cancer cells lead to early peritoneal metastasis. Methods： HMrSV5 cells were co - incubated with the supematants of gastric cancer cells. Morphological changes of HMrSV5 cells were observed. The cell damage was quantitatively determined by MTF assay. The apoptosis of HMrSV5 cells was observed under transmission electron microscope. Acridine orange/ethidium bromide - stained condensed nuclei was detected by fluorescent microscopy and flow cytometry. The expressions of Bel - 2 and Bax was immunochemieally evaluated. Results： Conspicuous morphological changes of apoptosis were observed in HMrSV5 cells 24 h after treatment with the supernatants of gastric cancer cells. The supematants could induce apoptosis of HMrSV5 cells in a time - dependent manner. The supernatants could up - regulate the expression of Bax and suppress that of Bel -2 in HMrSV5 cells. Conclusion： Gastric cancer cells can induce the apoptosis of HPMCs through supernatants in the early peritoneal metastasis. The abnormal expressions of Bel -2 and Bax may contribute to the apoptosis.

关 键 词：腹膜癌转移 胃癌 间皮细胞 细胞凋亡 

分 类 号：R730.23[医药卫生—肿瘤] R735.2[医药卫生—临床医学]