p53负向调控肺癌A549细胞Wnt通路活性并抑制其增殖和侵袭  

p53 negatively regulates Wnt pathway and inhibits the proliferation and invasion of lung cancer cell

作　　者：杨连赫[1] 马爽[2] 王健[1] 张清富[1] 崔权哲[1] 唐仲萍[1] 徐中海[1] 王恩华[1] 

机构地区：[1]中国医科大学基础医学院病理学教研室,中国医科大学附属第一医院病理科,辽宁沈阳110001 [2]中国医科大学附属盛京医院神经内科,辽宁沈阳110004

出　　处：《现代肿瘤医学》2013年第2期253-256,共4页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:No.81071905);教育部博士点基金资助项目(编号:No.20102104110015)

摘　　要：目的:探讨野生型p53对Wnt通路的调节以及对肺癌细胞生物学行为的影响。方法:分别利用野生型p53质粒及siRNA,分别转染和干扰A549细胞中的野生型p53,而后用Western blot检测A549细胞中β-catenin和cyclin D1的表达,用荧光素酶报告基因法检测Wnt通路活性,用MTT和Transwell实验检测A549细胞的增殖和侵袭能力。结果:转染野生型p53后能够明显下调A549细胞中β-catenin和cyclin D1表达(P<0.01),抑制肺癌细胞的增殖和侵袭(P<0.05),而干扰p53可以明显地上调β-catenin和cyclin D1表达(P<0.01),增强肺癌细胞的增殖力和侵袭力(P<0.05)。结论:野生型p53负向调控A549细胞中的Wnt信号转导通路,抑制其增殖和侵袭能力。Objective：p53 is one of the most important tumor suppressors, the regulation of wild - type p53 on Wnt pathway in lung cancer cells is still not clear, we explore the wild type p53 function on Wnt pathway and lung cancer cell biology behaviors. Methods ： Wild - type p53 plasmid and siRNA were used to transfect and knock down p53 in A549 cells,Western blot was used to detect β -catenin and cyclin D1 protein level, luciferase reporter gene was used to detect the activity of the Wnt pathway, MT/＇ and Transwell assay were used to detect the proliferation and invasion of A549 cells. Results： The transfection of wild - type p53 markedly reduced β - catenin and cyclin D1 in A549 cells （ P 〈0.01 ） and inhibited the proliferation and invasion （P 〈0.05 ） ,knock down of p53 up - regulated β - catenin and cyclin D1 （ P 〈 0.01 ）, and enhanced proliferation and invasion in A549 cells （ P 〈 0.05 ）. Conclusion： The wild -type p53 negatively regulates Wnt signaling transduction pathway and inhibits the proliferation and invasion of A549 cells.

关 键 词：P53 Β-连环蛋白 肺癌 

分 类 号：R730[医药卫生—肿瘤] R734.2[医药卫生—临床医学]