MDR1基因多态性与其在乳腺癌患者中表达水平的关系  

Association of MDR1 polymorphism with mRNA expression in breast cancer patients

作　　者：刘学敏[1,2] 王树生[1] 

机构地区：[1]南京中医药大学附属张家港市中医院普通外科,江苏张家港215600 [2]南京医科大学附属肿瘤医院,江苏南京210009

出　　处：《现代肿瘤医学》2013年第2期319-321,共3页Journal of Modern Oncology

摘　　要：目的:探讨MDR1基因多态性与其在乳腺癌患者癌组织中表达水平的关系。方法:筛选93例乳腺癌组织及26例配对癌旁组织,利用PCR-RFLP技术检测MDR1 exon12(1236)、exon21(2677)、exon26(3435)3个位点的多态性,以RT-qPCR技术对MDR1 mRNA进行相对定量,分析不同基因型患者间MDR1基因的表达水平。结果:93例乳腺癌组织中均有不同程度MDR1基因的表达;C1236T、G2677T/A和C3435T各基因型与MDR1表达水平之间均未显示统计学差异(F=0.047,P=0.954;F=0.364,P=0.833和F=0.173,P=0.841);但其中2 6例癌组织MDR1 mRNA表达水平,明显高于其对应的癌旁组织(3.83±5.27 vs 1.81±4.42;t=2.522,P=0.018)。结论:癌组织可能易发生多药耐药,MDR1表达差异的遗传学基础还有待进一步研究。Objective：To investigate whether MDR1 polymorphisms are associated with expression in breast cancer patients, nethods：PCR- RFLP technique was used for the detection of MDR1 variants in 93 patients＇ blood samples. We detemined the MDR1 mRNA expression using real - time PCR in 93 tumor tissues and 26 nontumor tissues. Results：There was lack of association of C1236T and G2677T/A as well as C3435T polymorphisms on MDR1 mRNA expression in breast cancer patients. MDR1 mRNA expression in tumor tissues revealed a significantly higher level than in nontumor tissues. Conclusion：We cannot exclude an association of MDR1 variants on mRNA expression.

关 键 词：乳腺癌 多药耐药基因1(MDR1) 多态性 表达 

分 类 号：R737.9[医药卫生—肿瘤]