GPC-3 mRNA与GGT联合检测诊断AFP阴性原发性肝细胞肝癌  

Combination detection of GPC-3 mRNA and GGT for diagnosis of AFP-negative hepatocellular carcinoma

作　　者：范钦桥[1] 陈书伟[1] 陈小雪[1] 杨正[1] 袁超[1] 黄军[1] 

机构地区：[1]湖南省郴州市第一人民医院/南院肝胆外科二区,湖南郴州423000

出　　处：《中国普通外科杂志》2013年第1期59-62,共4页China Journal of General Surgery

基　　金：湘南学院立项课题(09Y033)

摘　　要：目的:探讨外周血单核细胞磷酸肌醇蛋白聚糖3(GPC-3)mRNA联合血浆γ-谷氨酰转肽酶(GGT)检测诊断甲胎蛋白(AFP)阴性原发性肝细胞肝癌(HCC)的价值。方法:采用RT-PCR方法分别检测39例AFP阴性HCC患者、39例非HCC肝病患者及40例健康自愿者外周血单核细胞GPC-3 mRNA的表达;用全自动生化分析仪检测39例AFP阴性HCC患者血浆GGT水平(>58 U/L为阳性),分析两者单独和联合检测阳性率的差异。结果:39例HCC患者外周血单核细胞GPC-3 mRNA表达检出率为62%(24/39),而在非HCC的肝病患者及健康对照者外周血单核细胞中GPC-3 mRNA表达均为阴性,特异度达100%;39例HCC患者血浆GGT升高阳性率80%(31/39)。GPC-3 mRNA联合GGT检测的阳性率95%,明显高于两者单独检测的阳性率(均P<0.05)。结论:AFP阴性HCC患者外周血单核细胞中GPC-3 mRNA有较高的特异性表达,GPC-3 mRNA联合GGT检测有助于AFP阴性的HCC早期发现。Objective： To investigate the diagnostic value of the combination detection of the glypican 3 （GPC-3） mRNA expression in peripheral blood monouclear cells （PBMC） and plasma level of）,-glutamyl transpeptidase （GGT） for patients with a-fetoprotein （AFP）-negative hepatocellular carcinoma （HCC）. Methods： The PBMC GPC-3 mRNA expressions in 39 patients with AFP-negative HCC, 39 patients with non- HCC liver diseases and 40 healthy volunteers were determined by RT-PCR method, and the plasma GGT levels（〉58 U/L was considered as positive） in the 39 AFP-negative HCC cases were measured by using automatic biochemical analyzer. The positive rates by single or combination of the two detections were analyzed. Results： The detection rate of PBMC GPC-3 mRNA expression in the 39 AFP-negative HCC patients was 62% （24/39）, while none of the cases in patients with non-HCC liver diseases and healthy subjects presented positive expression of PBMC GPC-3 mRNA, thus the specificity of PBMC GPC-3 mRNA for AFP-negative HCC was 100%. The positive rate of GGT elevation in the 39 AFP-negative HCC patients was 80% （31/39）. The positive rate of the combination detection of PBMC GPC-3 mRNA and GGT reached 95%, which was significantly higher than that of either GPC-3 mRNA or GGT alone （both P〈0.05）. Conclusion： PBMC GPC-3 mRNA expression has a high specificity in AFP-negative HCC patients. The combination detection ofGPC-3 mRNA and GGT is helpful to the early diagnosis ofAFP-negative HCC.

关 键 词：癌 肝细胞 诊断 磷脂酰肌醇蛋白聚糖类 Γ-谷氨酰转移酶 

分 类 号：R735.7[医药卫生—肿瘤]