LKB1在甲状腺乳头状癌中表达及意义  

LKB1 Expression in Human Papillary Carcinoma of Thyroid and Its Biological Significance

作　　者：沈峰清[1] 姚军[2] 杨晶金[1,2] 杨林军[3] 金晓燕[3] 梁勇[2,3] 

机构地区：[1]温州医学院第一临床医学院肿瘤科,325000 [2]台州学院医学院,318000 [3]台州学院医学院附属市立医院肿瘤科,318000

出　　处：《医学研究杂志》2013年第1期26-30,共5页Journal of Medical Research

基　　金：国家自然科学基金资助项目(81072209);台州市科技计划项目(102ky11)

摘　　要：目的探讨LKB1在甲状腺乳头状癌中的表达特点及其生物学意义。方法甲状腺乳头状癌和正常甲状腺组织标本各25例,采用免疫组织化学、Western blot和real-time PCR检测比较LKB1蛋白表达及mRNA情况。结果人甲状腺乳头状癌组织中LKB1蛋白表达显著低于正常甲状腺组织(P<0.05),其mRNA明显下调(P<0.01),LKB1表达与甲状腺乳头状癌淋巴结转移呈负相关(P<0.01)。结论 LKB1基因和蛋白表达水平在甲状腺乳头状癌组织中普遍下调并与其淋巴结转移呈负相关,提示LKB1在甲状腺乳头状癌的形成及淋巴结转移中起负向调节作用。Objective To compare the mRNA and protein expression of LKB1 in the papillary thyroid carcinoma and normal thyroid tissue, and investigate its biological significance. Methods LKB1 protein was detected by immunohistochemistry and Western blot in 25 cases of human papillary carcinoma of thyroid, 25 cases of normal thyroid tissues around the nodular goiter as control. The expression of LKB1 mRNA was detected by real - time PCR in above cases. Results Compared with normal thyroid tissue,LKB1 ＇s expression was significantly reduced both in protein and mRNA level in papillary thyroid cancer（ P 〈0.05 ,P 〈 0.01 ） ,and reduction of LKB1 protein were reverse correlated with lymphatic metastasis （P 〈 0.01 ）. Conclusion LKB1 was lower expression in the papillary thyroid carcinoma both in gene and protein level, suggesting that LKBI plays a negative regulation role in the formation of papillary thyroid carcinoma and lymph node metastasis.

关 键 词：LKB1 甲状腺乳头状癌 免疫组织化学 WESTERN BOLT REAL-TIME PCR 

分 类 号：R736.1[医药卫生—肿瘤]