心力衰竭大鼠模型制备和评估  

Preparation and Evaluation of RAT Experimental Model of Heart Failure

作　　者：魏罡[1] 田国忠[1] 李梅秀[1] 魏伟 牛莹莹[3] 徐建博[1] 徐振宇[1] 

机构地区：[1]佳木斯大学基础学院局部解剖学教研室,154007 [2]商洛职业技术学校 [3]牡丹江医学院公共卫生学院

出　　处：《医学研究杂志》2013年第1期79-83,共5页Journal of Medical Research

基　　金：黑龙江省自然科学基金资助项目(D2006-24);黑龙江省教育厅研究生创新科研项目(YJSCX2011-373HLJ)

摘　　要：目的建立一种稳定可重复的大鼠心力衰竭(heart failure、HF)模型。方法 Wistar大鼠经麻醉后自主呼吸,结扎大鼠左前降支诱导HF模型。术后8周末行超声心动图,Masson及HE染色病理学检查。结果组织病理学证实大鼠HF模型建立成功,取材均见心脏增大,左心室壁呈灰白色,室壁瘤形成等。Masson染色、HE染色病理组织学观测,见大量炎性细胞浸润,纤维组织增生,肺组织淤血,证明结扎位置准确造模成功。心脏超声提示:HF组8周末较假手术组心脏增大明显,左心室舒张末期内径、左心室收缩末期内径明显增加,差异具有统学计学意义(P<0.001)。HF组较假手术组舒张末期室间隔厚度及左心室厚壁厚度显著降低,射血分数值比较显著降低,差异有统计学意义(P<0.001),表明结扎左前降支诱导HF模型出现收缩功能下降,符合HF表现,模型构建成功。采用自主呼吸,结扎左前降支诱导HF模型,8周末手术成活率为64.29%。结论本实验方法具有操作简单易行、可复制性强、成活率高的特点,是理想的HF模型,对HF基础实验研究具有具有重要意义。Objective To establish a steady and repeatable rat heart failure （heart failure, HF） model. Methods Wistar rats after the anesthesia, spontaneous breathing, ligaturing the left before descending branch of the rat caused extensive myocardial infarction, induced heart failure mode. And then we use ultrasound electrocardiogram, Masson and HE dyeing pathology examination to detected the rats which were 8 weeks after surgery. Results Through the biopsy, prove the model building success. Such as heart increases, the left ventricular wall are pale, ventricular aneurysim were found in those rats＇ Heart tissues. Through the Masson dyeing, HE dyeing, note the amount of inflammatory cells infiltration, fibre hyperplasia, lung tissue passive congestion, these results prove ligation position accurate, model manufacturing success. Echocardiogram tip： Eight weeks after the operation model of HF group rat＇ hearts increase significantly. In end - diastolic, HF rats inside diameter of left ventricular are increased and Left ventricular end - systolic significantly than sham operated group rats. Difference has statistics significance（P 〈 0. 001 ）. The left ventricular wall of HF group was thinner ejection fraction and left ventricular septal thick wall was significantly lower than sham operated group rats. Difference has statistics significance （ P 〈 O. 001 ）. Spontaneous breathing and ligaturing left before descending branch the methods make 8 week survival rate of postoperative rats reached 64.29%. Conclusion The method has simple operation, repeatability of strong, the characteristics of high survival rate which is the ideal HF model, This model based on experimental research HF has important significance.

关 键 词：心肌梗死 心力衰竭 大鼠 动物模型 

分 类 号：R-332[医药卫生]