T细胞亚群和自然杀伤性细胞活性在脑胶质瘤患者中表达的意义  

Determination of T Cell Subgroups and NK Cells in Peripheral Blood of Glioma Patients and Its Clinical Significance

作　　者：陈贤斌[1] 涂明[1] 王成德[1] 王旭阳[1] 朱丹化[1] 李浩旭[2] 

机构地区：[1]温州医学院附属第一医院神经外科,325000 [2]西安医学院解剖学教研室,710021

出　　处：《医学研究杂志》2013年第1期90-91,124,共3页Journal of Medical Research

基　　金：陕西省卫生厅基金资助项目(06E05)

摘　　要：目的检测脑胶质瘤患者外周血T淋巴细胞亚群、自然杀伤(NK)细胞活性的水平,探讨其与良、恶性脑胶质瘤的关系,并探讨与神经功能状态KRS计分有无相关。方法 44例脑胶质瘤患者根据相应判定标准分为良性组和恶性组,设立健康对照组44例,采用流式细胞仪检测所有实验者的血清T淋巴细胞亚群、NK细胞活性水平。结果脑胶质瘤患者CD4+细胞值显著降低、CD8+细胞值则显著升高。CD4+/CD8+比值及NK细胞活性均显著降低。恶性组与良性组相比,CD4+细胞值、CD4+/CD8+比值及NK细胞活性(NKA)也均显著降低。CD4+/CD8+比值和NK细胞活性与KRS计分有相关性。结论脑胶质瘤患者免疫功能低下及NK活性降低与肿瘤的恶性程度有关。T细胞亚群及NK细胞活性与神经功能状态KRS计分呈正相关。Objective To detect lymphocyte T subgroups and NK cells in peripheral blood of patients with glioma,and to investigate the relation between the immune function and the malignant and benign disease. Methods Lymphocyte T subgroups and NK cells in peripheral blood of 44 glioma patients and 44 healthy people were detected with flow cytometry. Results The amounts of T cell CD4 ＋ , the ratios of CD4 ＋/CD8 ＋ , and NK cytotoxieity were significantly higher,and the amounts of T cell CD8 ＋ were significantly lower in glioma patients than those in control group. These changes became more obvious in the malignant disease . It was highly correlative between CD4 ＋/CD8 ＋ , NK cells and KRS. Conclusion Cell immune function disorder occurs in glioma patients,and becomes more severe along with the development of disease. These changes might provide important information for the diagnosis of glioma in clinic.

关 键 词：脑胶质瘤 T淋巴细胞亚群 NK细胞活性 流式细胞术 

分 类 号：R739.41[医药卫生—肿瘤]