烟酸对实验性动脉粥样硬化兔主动脉骨桥蛋白表达的影响  

Effect of Nicotinic Acid on Plaques Formation and Expression of Osteopontin mRNA in Aorta of Experimental Atherosclerosis Rabbit

作　　者：文伟明[1] 雷敏[2] 徐戈[1] 李志乐[1] 

机构地区：[1]广西医科大学第一附属医院心血管病研究所,广西南宁市530021 [2]广西玉林市红十字会医院心内科,广西玉林市537000

出　　处：《中国动脉硬化杂志》2013年第2期149-153,共5页Chinese Journal of Arteriosclerosis

基　　金：广西青年科学基金资助(桂科青0832049)

摘　　要：目的探讨烟酸对动脉粥样硬化兔主动脉壁骨桥蛋白表达的影响。方法 16只雄性新西兰大白兔给予高脂饮食8周后,随机分为高脂血症组与烟酸组:高脂血症组(n=8)继续饲以高脂饲料6周;烟酸组(n=8)在高脂饮食基础上给予烟酸[200 mg/(kg.d)]6周。另选8只兔给予普通饮食14周作为正常对照组。14周末处死动物进行主动脉病理学检测,采用免疫组织化学染色检测兔主动脉壁骨桥蛋白的表达水平,采用实时定量PCR检测各组兔主动脉壁骨桥蛋白mRNA的表达。结果与正常对照组相比,高脂血症组主动脉内膜厚度和斑块面积显著增加,骨桥蛋白和mRNA表达显著增加(P<0.01)。烟酸组主动脉内膜和斑块面积显著缩小,骨桥蛋白和mRNA表达显著减少(均P<0.01)。相关性分析显示:骨桥蛋白表达量与动脉粥样硬化斑块面积(r=0.821,P<0.01)及内膜厚度(r=0.818,P<0.01)均呈正相关;骨桥蛋白mRNA表达量与动脉粥样硬化斑块面积(r=0.888,P<0.01)及内膜厚度(r=0.874,P<0.01)也均呈正相关。结论烟酸抗动脉粥样硬化作用除与其降脂作用有关外,还可能与其降低骨桥蛋白和mRNA水平有关。Aim To investigate the effects of nicotinic acid on the atherosclerotic plaques formation and osteopontin （OPN） mRNA expression of aorta. Methods 16 New Zealand white rabbits were fed with high-fat diet for 8 weeks, and then were randomly divided into two groups ： （ 1 ） hyperlipidemia group （ n = 8 ） ： maintained high-fat diet for 6 weeks ; （2） niacin treatment group （ n = 8） ： the same fat diet supplemented with niacin [ 200 mg/（ kg · d） ] for 6 weeks. Control group （ n = 8） was fed with normal diet for 14 weeks. At the end of the study, all rabbits were sacrificed to detect the histopathological changes and OPN mRNA expression of the aortas. Results Rabbits fed with high-fat diet showed more aorta endometrial thickness, plaque area and OPN mRNA expression （ all P 〈 0. 01 ）. Compared with hyperlipidemia group, rabbits treated with niacin showed less aorta endometrial thickness, plaque area and OPN expression （ all P 〈 0. 01 ）. Correlation analysis suggested that expressions of OPN mRNA （ r = 0. 888, P 〈 0. 0! ） and OPN protein （ r = 0. 821, P 〈 0. 01 ） were positively correlated with area of atherosclerotic plaque, expressions of OPN mRNA （ r = 0. 874, P 〈 0. 01 ） and OPN protein （ r = 0. 818, P 〈 0. 01 ） were also positively correlated with intimal thickness of aorta. Con- clusion Niacin＇ s role of anti-atherosclerosis not only is due to its lipid-lowering effect, but also may be related to its OPN expression-lowering effect.

关 键 词：烟酸 动脉粥样硬化 骨桥蛋白 

分 类 号：R363[医药卫生—病理学]