stTRAIL-MSC靶向促进肝癌大鼠RFA过渡区残癌细胞凋亡的体内研究  

stTRAIL-MSC targeting to promote liver cancer in rats the RFA the transition zone residual cancer cells to apoptosis in vivo research

作　　者：张哲菠[1,2] 邓青松[1] 熊青[1] 李勇[1] 周华[1] 刘念洲[1] 姜鹏[1] 马宽生[1] 李晓武[1] 别平[1] 

机构地区：[1]第三军医大学西南医院全军肝胆外科研究所,重庆400038 [2]中国人民解放军359医院外科,重庆400038

出　　处：《中国现代普通外科进展》2013年第1期5-10,共6页Chinese Journal of Current Advances in General Surgery

基　　金：国家自然科学基金(30972894;81272688)

摘　　要：目的:探讨stTRAIL-MSC靶向促进肝癌大鼠RFA过渡区残癌细胞凋亡的作用。方法:对150只雄性SD大鼠,采用N1S1肿瘤细胞建立肝荷瘤大鼠模型,并随机将其分为stSTRAL-MSC组、MSC组、Vector-MSC组、培养液组以及空白对照组,每组30只,在接受治疗前1周给以注射相应的制剂,在接受RFA治疗后12 h、24 h、48 h以及1周时每组取6只处死并进行相关检查(空白组除外),同时比较空白组与RFA组其生存时间。结果:stTRAIL-MSC在接受RFA治疗后其肿瘤体积变化、过渡区肿瘤细胞凋亡指数、大鼠肿瘤毁损体积、过渡区肿瘤细胞(ki-67染色)增殖指数等比较明显优于其他几组,而且在对caspase-3、caspase-8、Bcl-2水平检测时发现,stTRAIL-MSC其caspase-、3caspase-8水平明显高于其他四组,而Bcl-2水平低于其他四组,对接受RFA以及空白对照组大鼠的生存情况分析发现,采用RFA治疗的大鼠其生存时间明显高于空白对照组。结论:stTRAIL-MSC靶向通过提高cas-pase蛋白酶级联反应继而达到促进肝癌大鼠RFA过渡区残癌细胞凋亡。Objective： To investigate stTRAIL-MSC targeting to promote the role of residual cancer cells to apoptosis of liver cancer in rats RFA transition zone. Methods： 150 male Sprague-Dawley rats, using of N1S1 tumor cells to establish a rat model of liver tumor-bearing and randomly divided into stSTRAL-MSC group and MSC group, Vector-MSC group, medium group and control group, each group of 30, one week before receiving treatment give injections corresponding preparations 12 h underwent RFA treatment, 24 h, 48 h, and one week each group were sacrificed at 6 and checks （other than blank group）, while relatively blank group with RFA group whose survival time. Results： stTRAIL-MSC after RFA changes in tumor volume, tumor cell apoptosis transition zone index, rat tumor damaged volume, transition zone tumor cells （Ki-67 staining） proliferation index was significantly better than other groups, and found in caspase-3 and caspase-8, Bcl-2 levels detected, stTRAIL-MSC its caspase-3 and caspase-8 levels were significantly higher than the other four groups, the Bcl-2 level was lower than the other four groups, accept the RFA and blank control group rats survival analysis found that RFA treatment of rats survival time was significantly higher than the control group. Conclusion： stTRAIL-MSC targeted by caspase protease cascade reaction and then reached the RFA the transition area to promote liver cancer in rats residual cancer cells to apoptosis.

关 键 词：肝癌 射频消融·残癌·靶向治疗·干细胞·分泌型TRAIL三聚体 

分 类 号：R730.5[医药卫生—肿瘤]