藤茶总黄酮及黄酮醇类化合物的抗血栓作用研究  

Antithrombotic Effect of Total Flavonoids and Monomeric Compounds from Ampelopsis grossedentala

作　　者：叶勇[1,2] 欧贤红[3] 黄秋洁[4] 刘华钢[2] 宋云飞 

机构地区：[1]桂林莱茵生物科技股份有限公司博士后工作站,广西桂林541100 [2]广西医科大学药学院,广西南宁530021 [3]桂林医学院药学院,广西桂林541004 [4]广西中医药大学药学院,广西南宁530001

出　　处：《中药新药与临床药理》2013年第1期33-36,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广西教育厅基金(201010LX340)

摘　　要：目的研究藤茶提取物藤茶总黄酮(TF)、二氢杨梅素(DMY)及杨梅素(Myr)的抗凝血及溶栓作用。方法采用毛细玻璃管法观察对小鼠凝血时间的影响;采用下腔静脉结扎大鼠血栓模型,观察受试药物的抗血栓作用。结果与正常对照组比较,高剂量(2 mg.kg-1)的TF、DMY及Myr均能明显延长小鼠凝血时间(P<0.05,P<0.01);低剂量(0.5 mg.kg-1)的TF、DMY及Myr虽有延长小鼠凝血时间的趋势,但差异无统计学意义。下腔静脉结扎血栓模型结果显示,阳性药川芎嗪、Myr及DMY各组的血栓干重均明显低于正常对照组(P<0.01,P<0.05);Myr高剂量组的血栓湿重明显低于正常对照组(P<0.01),TF高、低剂量组的血栓湿重、干重与正常对照组比较,差异均无统计学意义。各实验组对血栓的抑制率大小依次为:Myr高剂量组>川芎嗪组>DMY低剂量组>Myr低剂量组>DMY高剂量组>TF高剂量组>TF低剂量组。结论 Myr和DMY可能是藤茶抗血栓作用的主要有效成分,且Myr的抗血栓作用稍强于DMY。Objective To study the anti-coagulation and thrombolytic effect of total flavonoids（TF）, dihydromyricetin （DMY）and myricetin （Myr）from Ampelopsis Grossedentala. Methods The effects on coagulation time in mice were observed by capillary glass tube method, and the antithrombotic effects were observed in mouse model of venous thrombosis induced by ligation of inferior vena cava. Results Compared with the normal group, high dose （2 mg·kg-1） of TF, DMY and Myr could significantly prolong the coagulation time of mice （P 〈 0.05, P 〈 0.01 ）, respec -tively. However, low dose（0.5 mg·kg-1）of TF, DMY and Myr had no significant effect on coagulation time of mice. In mouse model of venous thrombosis, positive drug （tetramethylpyrazine）, myricetin and dihydromyricetin could obviously reduce dry thrombus weight（P 〈 0.01, P 〈 0.05 compared with the normal group） , high dose of myricetin could obviously reduce wet thrombus weight（P 〈 0.01 ）. However, high- and low-dose TF groups had no significant effect on wet and dry thrombus weight compared with the normal group. The effect of each group on throm- bus inhibition ratio was as follows： 2 mg·kg-1 Myr〉 tetramethylpyrazine 〉 0.5 mg＇kg-L DMY〉0.5 mg·kg-1 Myr〉 2 mg· kg-1 DMY〉2 mg·kg-1 TF〉 0.5 mg·kg-1 TF. Conclusion Myr and DMY may be the main antithrombotic active ingredi- ent of flavonoids from A. grossedntata, and myricetin has stronger thrombolytic effect than dihydromyricetin.

关 键 词：藤茶 藤茶总黄酮 二氢杨梅素 杨梅素 抗凝血 抗血栓 

分 类 号：R285.5[医药卫生—中药学]