罗格列酮逆转丝裂霉素对人胃癌SGC7901/VCR细胞株耐药的作用  

The Influence of Rosiglitazone Reverses Mitomycin C Resistance on Human Gastric Cancer Cells Lines SGC7901/VCR

作　　者：胡剑峰[1,2] 张琍[2] 

机构地区：[1]南华大学附属第二医院消化内科,421001 [2]浏阳市人民医院消化内科,410300

出　　处：《实用癌症杂志》2013年第1期1-4,共4页The Practical Journal of Cancer

基　　金：湖南省自然科学基金项目(No.08JJ5002);湖南省科技厅基金(No.2009SK3138);湖南省卫生厅基金(No.2007110)项目

摘　　要：目的探讨过氧化物酶体增殖子活化受体γ(PPARγ)配体罗格列酮(ROS),对耐药胃癌SGC7901/VCR细胞药物敏感性的影响。方法以长春新碱(VCR)诱导的人胃癌多药耐药细胞SGC7901/VCR及其亲代SGC7901为研究对象,应用MTT比色法及集落形成实验,研究罗格列酮对丝裂霉素(MMC)抑制人胃癌SGC7901细胞及其耐药亚系SGC7901/VCR细胞生长及增殖的影响及罗格列酮逆转SGC7901/VCR细胞对MMC的耐药作用。结果 ROS对SGC7901/VCR及SGC7901细胞生长具有明显抑制作用,且其作用与ROS浓度呈时间-剂量依赖关系;40μmmol/L、80μmmol/L ROS逆转SGC7901/VCR细胞对MMC的耐药倍数(RI)分别为9.6、10.5,与增敏对照组作用相当(P>0.05);集落形成实验结果显示,ROS与MMC联用降低SGC7901/VCR细胞的集落形成率。结论罗格列酮能部分逆转多药耐药胃癌细胞株SGC7901/VCR对MMC的耐药。Objective To explore the influence of Rosiglitazone（ROS）,a selective Peroxisome proliferator-activated receptor gamma（PPARγ） ligand,on the drug sensitivity of drug-resistant gastric cancer cell line SGC7901/VCR.Methods MTT assay and clone formation rate were used to determine the influence of ROS on Mitomycin C（MMC）-inhibited proliferation of gastric cancer cell lines SGC7901 and SGC7901/VCR.MTT assay was used to determine the reversal effect of ROS on multidrug-resistant human gastric cancer cell line SGC7901/VCR.Results ROS had inhibitory effects on the growths of human gastric cancer SGC7901/VCR cell line and its parental generation SGC7901 cell line,and effects were dose-dependent;The reserving multiple of ROS at 40、80 μmmol/L to SGC7901/VCR cell line were 9.6、10.5 respectively;Clone formation rate illustrated that ROS combined with MMC can decrease the colon formation rate of SGC7901/VCR.Conclusion ROS has some influence on reversing multi-drug resistance of gastric cancer cell line SGC7901/VCR.

关 键 词：过氧化物酶体增殖子活化受体γ 罗格列酮 多药耐药 SGC7901 VCR细胞株 

分 类 号：R73-36[医药卫生—肿瘤]