宫颈癌中cIAP1与Dab2的基因改变  

Preliminary Study of Genetic Change of cIAP1 and Dab2 in Cervical Carcinoma

作　　者：郑晓娟[1,2] 胡新荣[2] 唐泽立[2] 李飞虹[2] 黄冰[2] 庞天云[3] 

机构地区：[1]江苏省昆山市第一人民医院病理科,215300 [2]广东医学院病理教研室,524023 [3]广东医学院附属医院妇产科,524000

出　　处：《实用癌症杂志》2013年第1期45-48,共4页The Practical Journal of Cancer

摘　　要：目的通过检测cIAP1与Dab2基因在宫颈癌中是否有扩增或丢失,确定与宫颈癌发生发展相关的分子标志。方法对41例新鲜宫颈癌标本进行显微切割和DNA提取;PCR扩增宫颈癌DNA中的靶基因cIAP1与Dab2;运用bandscan 4.3对PCR扩增产物的电泳条带进行灰度扫描,并用软件SPSS13.0对数据进行处理,确定发生扩增或丢失的基因。结果在宫颈癌中,cIAP1与Dab2均有不同程度的扩增,宫颈癌组织中cIAP1相对灰度的均值为0.716,正常宫颈组织相对灰度的均值为0.550;宫颈癌组织中Dab2相对灰度值的均值为0.235,正常宫颈组织相对灰度的均值为0.189。癌组织较正常组织中cIAP1与Dab2的拷贝数明显增加,两者相比差异有统计学意义(P<0.05)。结论 cIAP1与Dab2在宫颈癌中均表现为扩增,提示两者均可作为宫颈癌发生发展的分子标志物。Objective To determine the relevant molecular markers in the development of cervical carcinoma by detecting whether cIAP1 and Dab2 amplify or delete obviously in cervical carcinoma.Methods Microdissection and DNA extraction were used in 41 cases of fresh cervical carcinoma specimens.cIAP1 and Dab2 were amplified through PCR method,which were target genes of cervical carcinoma.Bandscan 4.3 was used to scan the PCR product bands electrophoresed on the gels.SPSS13.0 was used to analyze the scanning data and determine the gene amplification or deletion.Results cIAP1 and Dab2 were both amplified in different extent in carcinoma.Average of relative greyscale of cIAP1 was 0.716 in cervical carcinoma,and 0.550 in normal cervical tissues.The corresponding average of Dab2 were 0.235 and 0.189,respectively.Copy numbers of cIAP1 and Dab2 increased obviously in carcinoma tissues than normal tissues.There was significant difference between the two groups（P0.05）.Conclusion cIAP1 and Dab2 show amplification in cervical carcinoma.This result suggests that cIAP1 and Dab2 may be valuable molecular markers in the development of cervical carcinoma.

关 键 词：宫颈癌 cIAP1基因 Dab2基因 显微切割 PCR 

分 类 号：R737.33[医药卫生—肿瘤]