江黄颡鱼小肠结肠炎耶尔森氏菌的主要生物学特性研究  

Major biological characteristics of Yersinia enterocolitica isolated from Pseudobagrus vachelli

作　　者：赵静[1] 王利[1] 邱翔[1] 苟小兰[1] 龙钟明[1] 王海娟[1] 

机构地区：[1]西南民族大学生命科学与技术学院动物遗传育种国家民委-教育部重点实验室,成都610041

出　　处：《西南民族大学学报（自然科学版）》2013年第1期12-16,共5页Journal of Southwest Minzu University(Natural Science Edition)

基　　金：四川省科技厅应用基础项目(2011JY0095);西南民族大学研究生创新型科研项目(CX2013SP81);四川省科技创新苗子工程项目(2012ZZ071);西南民族大学研究生学位点建设项目(2011XWD-SO71007)

摘　　要：从发病江黄颡鱼中分离出菌株YER6022,通过细菌培养、生理生化试、16SrDNA基因扩增等方法对其生物学特性进行研究.结果表明:该菌株为革兰氏阴性球杆菌,具有动力性,不发酵山梨醇,赖氨酸、苯丙氨酸阴性,尿素、鸟氨酸阳性.16SrDNA片段测序为1408bp,与小肠结肠炎耶尔森氏菌相似性达99%,GenBank登录号为JX434637.系统进化树显示菌株YER6022与耶尔森菌属的小肠结肠炎耶尔森菌亲缘关系最近.Strain-YER6022 is isolated from infected Pseudobagrus vachelli. Its biological characteristics and 16SrDNA gene are studied using physiological and biochemical tests and molecular biotechnology. The results show that it is Gram negative coccobacillus, with dynamic in nature, sorbitol not ferment, lysine and phenylalanine negative, urea and ornithine positive. The sequence of partial 16SrDNA gene is 1408bp, which shows 99% homology with Y. enterocolitica. Its GenBank accession number is JX434637. Phylogenetic tree shows that the isolated strain and Yersinia enterocolitica gather into one category naturally.

关 键 词：小肠结肠炎耶尔森氏菌 生物学特性 江黄颡鱼 

分 类 号：S852.6[农业科学—基础兽医学]