生物除磷系统中聚磷菌检测常用技术  

Commonly Used Technologies for Detecting Phosphate Accumulating Organisms in Biological Phosphorus Removal System

作　　者：潘丹华[1] 李勇[1] 张娜[1] 

机构地区：[1]苏州科技学院环境科学与工程学院,江苏苏州215011

出　　处：《环境科学与技术》2013年第1期79-83,共5页Environmental Science & Technology

摘　　要：作为生物除磷系统中的主要功能菌,对聚磷菌菌群进行定性、定量分析是深入理解生物除磷系统、提高除磷效率的必然趋势。目前常用于检测聚磷菌的方法主要有生物化学法和分子生物学法,文章主要阐述了荧光原位杂交技术、聚合酶链反应技术、变性梯度凝胶电泳技术以及多技术结合使用的特点及应用情况,并在此基础上提出了改进措施以及聚磷菌检测技术的发展方向。As the main functional bacteria in biological phosphorus removal system,it was an inevitable trend to have a qualitative and quantitative analysis for phosphate accumulating organisms（PAOs） in order to deeply understand the biological phosphorus removal system and improve phosphorus removal efficiency.Mainly used methods for detecting PAOs include chemical and molecular biological technologies.Characteristics and applications of technologies such as fluorescence in situ hybridization（FISH）,polymerase chain reaction（PCR）,degeneration gradient electrophoresis（DGGE）and combined technologies used in detecting PAOs were reviewed.Some improvement measures and future direction for PAOs detecting technologies were also offered.

关 键 词：聚磷菌 荧光原位杂交 聚合酶链反应 变性梯度电泳 

分 类 号：X172[环境科学与工程—环境科学]