褪黑素对小鼠视交叉上核c-fos基因表达的影响  

Effect of melatonin on the c-fos gene expression in suprachiasmatic nuclear of mice

作　　者：霍艳[1] 李经才[2] 江岩[3] 刘佳[3] 李宏[3] 

机构地区：[1]中国药品生物制品检定所毒理室,北京100050 [2]沈阳药科大学时间药理研究室,沈阳110015 [3]沈阳医学院分子生物学研究室,沈阳110031

出　　处：《中国药理学通报》2000年第3期261-264,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目!No 39570 82 0

摘　　要：目的　观察褪黑素对幼龄、老龄及光制颠倒条件下小鼠视交叉上核内c fos基因蛋白和mRNA水平的影响。方法　采用冰冻切片免疫组织化学染色法 ,观察褪黑素对小鼠视交叉上核c fos蛋白水平的影响 ;采用RT PCR法 ,扩增视交叉上核内c fosmRNA ,用琼脂糖凝胶电泳法检测扩增产物。结果　幼龄鼠c fos蛋白水平及mRNA水平均高于老龄鼠 ;光制颠倒条件下 ,c fos蛋白及mRNA水平均受抑制 ;给予褪黑素 ,c fos蛋白及c fosmRNA水平均提高。结论　c fos基因表达水平随年龄增加而降低 ;光制颠倒可抑制c fos基因的表达 ;施用褪黑素 ,c fos基因表达水平提高 ,说明褪黑素可对抗增龄及光制颠倒对c fos表达的抑制作用 ,从而发挥抗衰老作用。AIM To observe the c fos gene expression in the suprachiasmatic nuclear (SCN) of mice after melatonin(MEL) treatment under normal and light dark shifting circumstances. METHOD c fos production was determined by the immuno histochemical staining with the anti murine c fos antibody; A region selective RNA extraction and rapid RT PCR method was used to analyse mRNA level in the SCN. RESULTS The levels of c fos protein and mRNA in the young mice were higher than those in the old mice. In the light dark shifting (L:D 14:10, alternating every 3 days), both the levels of c fos protein and mRNA in each group were decreased. MEL was found to enhance their expression. CONCLUSIONS c fos expression is down regulated along with aging,Light dark shifting inhibits its expression, MEL(sc) administration can stimulate c fos expression even under the aging and light dark shifting conditions, Indicating that MEL might exert its anti aging effects through increasing c fos gene expression.

关 键 词：裉黑素 小鼠 视交叉上核 C-FOS 基因表达 抗衰老 

分 类 号：R977.1[医药卫生—药品]