四氯化碳诱导小鼠肝纤维化模型的建立  

A model of carbon tetrachloride-induced hepatic fibrosis in mice

作　　者：许建明[1] 徐叔云[2] 张运芳[2] 梅俏[2] 吴继锋[3] 

机构地区：[1]安徽医科大学第一附属医院消化科,合肥230022 [2]安徽医科大学临床药理研究所,合肥230022 [3]安徽医科大学病理教研室,合肥230022

出　　处：《中国药理学通报》2000年第3期339-341,共3页Chinese Pharmacological Bulletin

摘　　要：目的　建立四氯化碳诱导的小鼠肝纤维化动物模型。方法　用体积分数为 2 0 %四氯化碳皮下注射 3mon ,诱导昆明种小鼠肝纤维化形成。在造模 30d、6 0d、90d时 ,检测肝纤维化程度、肝脏羟脯氨酸和丙二醛含量、以及血清氨基转换酶和血清白蛋白水平。结果　小鼠肝脏纤维化程度和肝脏羟脯氨酸含量随造模时间延长而逐渐增加 (P <0 0 5～0 0 1) ,肝匀浆丙二醛含量和血清丙氨酸氨基转换酶明显升高 (P <0 0 1) ,造模后期血清白蛋白比例下降 (P <0 0 1)。结论　长期给予四氯化碳可导致小鼠肝纤维化形成 ,并有较明显的阶段性变化。AIM To establish a model of carbon tetrachloride\|induced hepatic fibrosis in mice. METHODS\ Chronic adminstration of 20% carbon tetrachloride (CCl 4) was carried out in Kun\|Ming mice for 3 months, and then the changes of hepatic pathology and index of hepatic biochemistry observed as an interval of 30 days between experiments respectively. RESULTS\ The score of hepatic fibrosis and the content of hepatic hydroxyproline were gradually increased ( P< 0 05～0 01). The content of molondiadehyde in liver monogenates and the activity of serumal aminotransferase were signifcantly higher than that of control groups in three observed stages ( P< 0 01) either, but the rate of albumin in serum was signifcantly reduced only at the end stage of hepatic fibrosis in mice induced by CCl 4 ( P< 0 05) CONCLUSION\ Chronic administration of CCl 4 to mice can induce histologically observable liver fibrosis with characteristic stages of hepatic fibrosis.

关 键 词：动物模型 肝纤维化 四氯化碳 病理 

分 类 号：R575.202[医药卫生—消化系统]