HPV16E6羧基端编码基因的克隆及真核细胞表达  

GENE CLONING AND EUKARYOTIC EXPRESSION OF HPV16E6 C-TERMINAL REGION

作　　者：陈华波[1] 赵蔚明[1] 于修平[1] 周亚滨[1] 卞继峰[1] 栾怡[1] 齐眉[1] 贾继辉[1] 董杰德[1] 

机构地区：[1]山东医科大学微生物学与免疫学教研室

出　　处：《山东医科大学学报》2000年第2期123-125,共3页Acta Academiae Medicinae Shandong

基　　金：山东省科委资助课题

摘　　要：目的 :探讨用人乳头瘤病毒 16型 (HPV16)早期基因E6羧基端基因片段 (E6C)研制DNA疫苗的可行性。方法 :采用PCR方法从质粒 pHPV16中获得E6C端基因片段 ,将其克隆入含巨细胞病毒(CMV)启动子的真核表达载体 ,脂质体介导基因转染LA795小鼠肺腺癌细胞并检测E6C的表达。结果 :成功构建了真核表达质粒 pLNCE6C ,免疫组化结果显示真核表达质粒pLNCE6C在LA795细胞中获得有效表达。结论 :选择去除转化功能区的E6C端基因构建真核表达质粒是一有益的尝试 。Objectives:To explore the feasibility of utilizing the gene fragment of Cterminal region of human papillomavirus type 16 E6(HPV16 E6)for DNA vaccine.Methods:E6C gene fragment was amplified from genome of pHPV16 by PCR,and cloned into eukaryotic plasmid pLNCX,then teansfected LA795 cell with the recombinant plasmid pLNCE6C using lipofectamine reagent.Results:The result showed recombinant pLNCE6C was successfully constructed and the E6 protein was detected by immunohistochemistry in the transfected cells.Conclusion:This study provides a good basis for further research on HPV16 E6C DNA vaccine.

关 键 词：乳头状瘤病毒 分子克隆 真核细胞 

分 类 号：R373.9[医药卫生—病原生物学] Q785[医药卫生—基础医学]