人子宫内膜癌裸鼠移植瘤株的建立及其生物学特性  

ESTABLISHMENT AND CHARACTERIZATION OF A XENOGRAFTED HUMAN ENDOMETRIAL CARCINOMA IN NUDE MICE

作　　者：赵兴波[1] 张义读[2] 苏应宽[1] 邴鲁军[2] 

机构地区：[1]山东省立医院妇产科 [2]山东医科大学组织学与胚胎学教研室

出　　处：《山东医科大学学报》2000年第2期194-197,共4页Acta Academiae Medicinae Shandong

摘　　要：目的 :建立人子宫内膜癌裸鼠移植瘤株 ,为开展子宫内膜癌的实验性研究奠定基础。方法 :将 3例子宫内膜癌手术标本移植于裸鼠皮下 ,生长后行鼠间连续传代 ,对移植瘤进行相关的生物学检测。结果 :3例标本 1例获得生长并行鼠间传代 ,历时 2 4个月建成人子宫内膜癌裸鼠移植瘤株EnCa 95V ,瘤株生长稳定 ,已传 2 1代 ,移植成瘤率 10 0 % ,光镜、电镜、免疫组化、流式细胞术检测移植瘤与患者原始肿瘤特征基本一致 ;染色体分析显示人类肿瘤染色体核型 ,众数为 4 3～ 92条。结论 :EnCa 95V是一株新的子宫内膜癌裸鼠移植瘤株 。Objective:In order to establish a xenografted tumour strain of human endometrial carcinoma in nude mice to provide a model in vivo for further investigation of this tumour.Metheods:Three human endometrial carcinomas derived from surgical specimens were transplanted into the nude mice.One graft was successively passaged after growing.A series of characteristics of the grafts were examined.Results:One of three tumors could be maintained by serial transplantation.The xenograft had been successively passaged 21 generations during a period of 24 months.A tumor strain EnCa95V was established,having a high rate of successful inoculation rate(100%) and growing steadly.The morphological and biolgical characteristics of its original tumor were preserved,The chromosomal analysis revealed an aneuploid human karyotype of 21～136 in number.The tumor markers and P 53 oncogenic product by immunocytochemical techniques showed high expression.Conclusions:The results showed that EnCa95V could be considered as a new xenograted tumor strain and may provide a model in vivo for further investigation of human endometrial carcinoma.

关 键 词：子宫肿瘤 肿瘤移植 子宫内膜癌 生物学 

分 类 号：R737.33[医药卫生—肿瘤] R73－37[医药卫生—临床医学]