短链酰基辅酶A脱氢酶在大鼠心脏发育中的表达及其与心肌肥厚的关系  

Expression of short-chain acyl-CoA dehydrogenase during heart development in rats and relationship with cardiac hypertrophy

作　　者：周四桂[1] 王平[2] 路遥[3] 袁茜[3] 臧林泉[1] 杨智承[1] 徐立朋[4] 

机构地区：[1]广东药学院临床药学系,广东广州510006 [2]深圳市药品检验所,广东深圳518029 [3]中山大学中山医学院 [4]暨南大学药学院新药研究所,广东广州510632

出　　处：《中国病理生理杂志》2013年第1期9-14,共6页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金青年基金资助项目(No.81000072;No.81001683);教育部留学回国人员启动基金资助项目(No.23610008);教育部博士学科点专项科研基金资助项目(No.20104401120003);广东药学院重点培养青年教师基金资助项目;广东省“十二五”医学重点学科(依托广东药学院附属第一医院、药科学院)

摘　　要：目的:研究大鼠心脏发育过程中短链酰基辅酶A脱氢酶(short-chain acyl-CoA dehydrogenase,SCAD)的表达变化规律,并探讨其与高血压大鼠心肌肥厚的关系。方法:观察不同时期Wistar大鼠和不同周龄自发性高血压大鼠心肌组织的SCAD蛋白表达及酶活性变化,检测大鼠的血清和心肌游离脂肪酸含量。结果:与胚胎期19 d Wistar大鼠组比较,出生后1 d、2周、6周及16周龄Wistar大鼠组心肌的SCAD蛋白表达及酶活性增加,血清和心肌游离脂肪酸含量明显减少,二者之间呈负相关,其中,从2周龄Wistar大鼠组开始差异有统计学意义。与周龄匹配的WKY大鼠组比较,2周龄自发性高血压大鼠组收缩压尚未升高,6周龄及16周龄自发性高血压大鼠组收缩压显著增高;各时点自发性高血压大鼠组的左室重量指数均明显增高,提示自发性高血压大鼠在血压升高之前,已经发生了明显的心肌肥厚。与周龄匹配的WKY大鼠组比较,2周、6周及16周龄自发性高血压大鼠组心肌的SCAD蛋白表达及酶活性明显下降,血清和心肌游离脂肪酸含量明显增加,呈显著负相关。结论:(1)SCAD蛋白表达随大鼠心脏的生长发育逐渐上调,可能与心脏对脂肪酸的利用增加密切相关。(2)SCAD的蛋白表达及其酶活性显著下降,可能是导致自发性高血压大鼠肥厚心肌能量代谢"胚胎型再演"的分子基础。AIM： To investigate the expression of short-chain acyl-CoA dehydrogenase during the heart deve- lopment in rats and to analyze the relationship between short-chain acyl-CoA dehydrogenase and cardiac hypertrophy in spontaneously hypertensive rats （SHR）. METHODS： The expression and activity of short-chain acyl-CoA dehydrogenase in the hearts of Wistar rats with different ages were measured. Free fatty acids in serum and cardiac muscles were also de- termined. RESULTS： Compared with the fetal rats of 19 d, the expression and activity of short-chain acyl-CoA dehydro- genase in the postnatal rats of 1 d, 2 weeks, 6 weeks and 16 weeks were increased, and free fatty acids in the serum and myocardium were obviously decreased. The difference began in evidence from the age of 2 weeks. The expression of short- chain acyl-CoA dehydrogenase was significantly up-regulated with negative correlation to free fatty acids in the serum and myocardium during heart development. Systolic blood pressure was similar in 2-week-old SHR and WKY rats, which signif- icantly increased in SHR of 6 weeks and 16 weeks old compared with the age-matched WKY rats. The ratio of left ventrieu-lar weight to body weight was markedly elevated in SHR of 2 weeks, 6 weeks and 16 weeks old compared with the age- matched WKY rats, indicating that the appearance of cardiac hypertrophy occurred before the development of hypertension in SHR. Compared with the age-matched WKY rats, the expression and activity of short-chain acyl-CoA dehydrogenase were decreased and free fatty acids in the serum and myocardium were obviously higher in SHR. The expression of short- chain acyl-CoA dehydrogenase was significantly down-regulated with a negative correlation to free fatty acids in the serum and myocardium of SHR. CONCLUSION： The expression of short-chain acyl-CoA dehydrogenase is increased during the heart development, which may be associated with the increase in cardiac fatty acid utilization. The down-regulated expres- sion of short-chain acyl-CoA dehydrog

关 键 词：短链酰基辅酶A脱氢酶 心脏发育 脂肪酸氧化 心肌肥厚 

分 类 号：R363[医药卫生—病理学]