软脉降脂合剂干预动脉搭桥术内皮细胞损伤的研究  

Effect of Ruanmaijiangzhi mixture intervention on endothelial cell function after artery bypass graft

作　　者：许平[1,2] 彭辉[3,4] 朱莹[3,4] 贺嵩敏[3,4] 冯兵[3,4] 李秀丽[5] 黄小千[5] 陈婷[6] 张小宁[7] 郑广娟[3,4] 

机构地区：[1]广东省心血管病研究所 [2]广东省人民医院,广东广州510080 [3]广东省中医院 [4]广东省中医药科学院,广东广州510006 [5]山东中医药大学,山东济南250355 [6]广州中医药大学,广东广州510006 [7]安徽省立友谊医院,安徽合肥230041

出　　处：《中国病理生理杂志》2013年第1期15-19,共5页Chinese Journal of Pathophysiology

基　　金：广东省自然科学基金资助项目(No.9151009504000007);高等学校博士学科点专项科研基金资助项目(No.20104425110005)

摘　　要：目的:观察软脉降脂合剂对兔动脉搭桥术后内皮细胞功能的影响,探讨软脉降脂合剂在动脉搭桥术后再狭窄发生过程中的防治作用,以及中药治疗冠状动脉搭桥术(CABG)后再狭窄的可能作用机制。方法:新西兰大白兔随机分为4组:模型组、阿司匹林对照组、软脉降脂中剂量组和软脉降脂高剂量组,行右侧颈动脉搭桥术。术后给药灌胃8周后,取抗凝血用流式细胞仪检测循环内皮细胞(CECs),取血清用ELISA检测P-选择素和细胞间黏附分子1(ICAM-1)水平。结果:各给药组和模型组比较,CECs相对比例、P-选择素和ICAM-1明显降低,差异有统计学意义(P<0.05)。软脉降脂合剂2组与阿司匹林对照组比较差异也有统计学意义(P<0.05)。结论:中药软脉降脂合剂能够降低动脉搭桥术后的CECs、P-选择素和ICAM-1水平,有助于改善CABG术后内皮细胞的功能。AIM： To observe the effect of Ruanmaijiangzhi mixture on the function of endothelial ceils in rab- bits after artery bypass graft. METHODS： A New Zealand white rabbit model of right common carotid artery bypass graft was established. The rabbits were randomly divided into 4 groups： model group, aspirin control group, middle dose of Ru- anmaijiangzhi group and high dose of Ruanmaijiangzhi group. Eight weeks after treatment, circulating endothelial ceils （CECs） in anticoagulated blood were detected by flow cytometry. The serum levels of P-selectin and intercellular adhesion molecule I （ ICAM-1 ） were measured by ELISA. RESULTS： Compared with model group, the relative ratio of CECs, and the levels of P-selectin and ICAM-1 in medication administration groups were significantly decreased. The effect in Ruan- maijiangzhi groups was better than that in aspirin control group. Ruanmaijiangzhi treatment showed a positive dose-related correlation. CONCLUSION： Chinese medicine Ruanmaijiangzhi mixture decreases CECs, P-selectin and ICAM-1 to im- prove the endothelial cell function after coronary artery bypass graft.

关 键 词：软脉降脂合剂 动脉搭桥术 内皮细胞 

分 类 号：R363[医药卫生—病理学]