跨种属研究Smad3在肝癌组织中的表达及意义  

Cross-species comparison of Smad3 expression in hepatocellular carcinoma tissues

作　　者：朱伶群[1,2] 曹骥[1] 卢晓旭[1,2] 李瑗[1] 欧超[1] 苏建家[1] 杨春[1] 

机构地区：[1]广西壮族自治区肿瘤防治研究所实验研究部 [2]广西医科大学研究生学院,广西南宁530021

出　　处：《中国病理生理杂志》2013年第1期70-75,共6页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30960428);广西自然科学基金资助项目(No.2012GXNSFAA053167)

摘　　要：目的:研究Smad3在人、大鼠、树鼩等不同种属的肝癌、癌旁及正常肝组织中的表达和意义,进一步验证跨种属筛选肝癌关键蛋白研究策略的可行性。方法:采用实时荧光定量PCR和Western blotting技术分别检测人、大鼠和树鼩的肝癌及其相应癌旁组织以及正常肝组织中Smad3 mRNA和蛋白表达水平。结果:Smad3 mRNA在人、大鼠和树鼩的肝癌中表达水平均低于其相应的癌旁组织(均P<0.05);在大鼠肝癌组织中的表达低于正常肝组织(P<0.05);在树鼩癌旁组织中的表达高于正常肝组织(P<0.05);其余各组织间mRNA表达水平的差别无统计学意义(均P>0.05)。Smad3蛋白在人和大鼠的肝癌组织中的表达水平均低于其相应的癌旁组织及正常肝组织(均P<0.05);在树鼩肝癌组织中的表达水平也低于相应的癌旁组织和正常肝组织,但差别无统计学意义(均P>0.05);3个种属的癌旁组织与正常肝组织比较,差别无统计学意义(均P>0.05)。结论:Smad3在人、大鼠和树鼩3个种属的肝癌组织中的mRNA和蛋白表达水平均下调,提示该蛋白表达水平的改变可能在肝癌发生发展中起重要作用,有可能成为防治肝癌的靶分子。AIM ： To study the expression of Smad3 and its significance in hepatocellular carcinoma （HCC） in different species including human, rat and tree shrew, and to verify the feasibility of cross-species oncogenomic approach. METHODS： Real-time fluorescent quantitative polymerase chain reaction and Western blotting were applied to detect the expression of Smad3 at mRNA and protein levels in HCC tissues, corresponding HCC adjacent liver tissues and normal liver tissues collected from different species including human, rat and tree shrew. RESULTS： The mRNA expression of Smad3 in HCC tissues of human, rat and tree shrew was lower than those in the corresponding HCC tissues adjacent liver tissues. The mRNA level of Smad3 in HCC tissues of rat was lower than those in its normal liver tissues, while that in HCC adjacent tissues of tree shrew appeared higher than those in the normal liver tissues. No significant difference of Smad3 expression in other tissues was observed. The protein levels of Smad3 in HCC of human and rat were lower than those in the correspond- ing HCC adjacent liver tissues and the normal liver tissues. However, the protein expression of Smad3 was at a low level in HCC tissues of tree shrew and was lower than that in the HCC adjacent liver tissues and the normal liver tissues, although without statistical difference. The differences of Smad3 expression between HCC adjacent liver tissues and normal liver tis- sues in all the 3 species were not statistically significant. CONCLUSION： Smad3 is lowly expressed in HCC tissues of dif- ferent species, suggesting that it might play a pivotal role in hepatocarcinogenesis and be applied as a key molecular target in HCC prevention and treatment.

关 键 词：跨种属 SMAD3蛋白 肝肿瘤 

分 类 号：R735.7[医药卫生—肿瘤]