NAFL大鼠Kupffer细胞表型分子表达及抗原提呈功能研究  

Study on phenotypes and antigen-presenting function of Kupffer cell from rats with nonalcoholic fatty liver

作　　者：张利利 向晓星 徐跃元 龚卫娟[2] 马莉 刘国芳 许秀华 

机构地区：[1]苏北人民医院消化307病区,江苏扬州225001 [2]扬州大学医学院免疫学教研室

出　　处：《胃肠病学和肝病学杂志》2013年第1期18-21,共4页Chinese Journal of Gastroenterology and Hepatology

摘　　要：目的探讨非酒精性脂肪肝(NAFL)大鼠Kupffer细胞(KCs)表型及抗原提呈功能。方法高脂饲料喂养建立NAFL大鼠,改良灌注消化法分离KCs,吞噬实验及免疫化学染色鉴定KCs,流式细胞仪检测KCs抗原提呈相关表型CD54、CD86和MHC-Ⅱ;KCs与淋巴细胞共培养,MTT法检测淋巴细胞增殖。结果灌注消化法分离大鼠KCs,纯度达98%;模型组KCs表面CD86分子、MHC-Ⅱ类表达较对照组增加(P<0.05),而CD54分子的表达虽有增加,但差异无统计学意义(P>0.05),模型组淋巴细胞促增殖能力较对照组明显增强(P<0.01)。结论 NAFL大鼠KCs的表型表达及抗原提呈功能增强,可能与NAFL发病有关。To investigate phenotypes and antigen-presenting function of Kupffer cells （KCs） from rats with nonalcoholic fatty liver （ NAFL）. Methods Rat NAFL model was established by high-fat diet. Rat KCs were isolated by improved perfusion digestion method and identified by phagocytosis experiments and immunocytochemistry. Flow cytometry （FCM） was used to observe KCs phenotype molecule CD54, CD86 and MHC-Ⅱ; KCs and lymphocytes were co-cultured and lymphocyte proliferation was measured by MTT assay. Results The purity of KCs isolated by perfusion digestion method was more than 98%. Compared with control group, the expression of KCs phenotypes molecule CD86,MHC-Ⅱ detected by FCM in model group increased significantly （P 〈 0.05 ） , while expression of molecule CD54 increased （ P 〉 0.05）. Lymphocyte proliferating ability of model group KCs increased obviously compared with the control group （ P 〈 0.01 ）. Conclusion Phenotypes molecule expression and antigen-presentation ability of KCs in NAFL rats are enhanced which may be involved in the pathogenesis of NAFL.

关 键 词：非酒精性脂肪肝 KUPFFER细胞 抗原提呈功能 细胞表型 大鼠 

分 类 号：R575[医药卫生—消化系统]